本研究選取影響仿生浸泡沉積法(biomimetic immersion)的因子進行重要性評估，分別為浸泡溫度、表面氧化鈦結構以及浸泡之溶液。選取10℃、37℃、80℃為本實驗之浸泡溫度；以體積濃度34%硝酸處理(HP)和高溫爐處理(HT)分別提供試片表面anatase、rutile混合結構和單純rutile結構的氧化鈦；以及選取模擬人體體液(simulated body fluid , SBF)和過飽和鈣溶液(supersaturated calcification solution , SCS)作為浸泡的溶液表面性質部份利用掃描式探針顯微鏡量測浸泡溶液後試片的表面粗糙度及型態；利用化學分析電子術和能量散佈光譜儀來做試片表面的化學成份的定量及定性分析；利用高解析場發掃描式電子顯微鏡觀察試片表面型態；利用多功能X光薄膜繞射儀分析試片表面的結晶型態，並將找出來的最佳條件，利用MMT、ALP、以及細胞表面型態來評估生物相容性。從SPM的結果發現浸泡溫度與試片表面粗糙度呈現正相關，當浸泡溫度越高時表面粗糙度越高；從FE-SEM表面型態也發現到浸泡溫度越高鈣磷化合物覆蓋面積也越大且由ESCA分析出的鈣磷含量也是隨浸泡溫度升高而增加；從浸泡後的表面粗糙度也發現經過硝酸表面鈍化所得的氧化鈦結構—anatase與rutile混合結構擁有較高的粗糙度。透過本實驗結果將浸泡溫度、試片表面氧化鈦結構以及浸泡溶液之重要性做評估，提供一個簡單且控制容易的仿生浸泡沉積法之最佳浸泡條件，且由生物相容性測試中可以發現，利用最佳浸泡條件的鍍層，對骨母細胞擁有良好的黏附及分化能力。

This study aims at investigating the optimal condition for HA coating in vitro by biomimetic immersion. In order to study the importance of the factors for deposited calcium phosphate apatite, it selected soaking temperature, immersing solutions, and the surface structure of Titanium dioxide, individually. The surface treatments of Ti6Al4V samples was carried out by passivating with heated in 400°C air (HT) and immersing in volume 34% nitric acid (HP) first .Then immersed them in simulated body fluid (SBF) or supersaturated calcification solution (SCS) at 10°C, 37°C or 80°C. The surface roughness and morphology is measured by Scanning Probe Microscope (SPM); the chemical property of surface deposition is analyzed by Electron Spectroscopy for Chemical Analysis (ESCA) and Energy Dispersive Spectrometer (EDS); the crystal of surface deposition is observed by Multipurpose X-Ray Thin-Film Diffractometer (TF-XRD); images of the calcium phosphate is taken by High Resolution Field-Emission Scanning Electron Microscopy (FE-SEM). Estimated the biocompatibility of the optinal immersed condition by using
MTT, alkaline phosphatase(ALP) ,and FE-SEM to observed the morphology of osteoblast

By using SPM and FE-SEM, it has been observed that increasing the soaking temperature would raise the surface roughness and the covered area of apatite. With ESCA analyzing, immersed at higher soaking temperature would increase the production of calcium phosphate apatite. In addition, the structure mixed by anatase and rutile had higher roughness than the pure structure of rutile. This study weighed the importance of factors and provided a simple and easily controllable optimal condition for biomimetic immersion.

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