環境中存在許多類雌激素物質，其可能會模擬天然雌激素之生理行為，造成許多假刺激的作用與過多的不當反應，並且會阻礙雌激素受體之正常鍵結而產生直接或間接之作用，對人體或動物造成繁殖能力降低、性別異常、癌症腫瘤罹患率增加之影響。
　　本研究利用基因重組酵母菌試驗法結合儀器分析方法檢測台灣嚴重污染河川之類雌激素物質分布情況，採取水樣包含淡水河、鹽水溪、二仁溪、阿公店溪及高屏溪共五條河川。
　　生物試驗法結果顯示二仁溪具有高度的類雌激素活性，也於淡水河、鹽水溪、阿公店溪與高屏溪測得中、高度的類雌激素活性。二仁溪之類雌激素污染以支流三爺溪最為嚴重，而淡水河之類雌激素污染分布於大漢溪與基隆河，鹽水溪之類雌激素污染分布於潭頂溪及鹽水溪中、下游，阿公店溪以中游發現較嚴重之污染，高屏溪則於支流測得較高活性。
　　液相層析串聯式質譜儀分析結果可知二仁溪中存在雙酚A、壬基酚、雌酮、雌三醇與乙烯雌酚等五種物質，其中以雙酚A與壬基酚濃度較高，但並非為主要貢獻類雌激素活性之物質。由前述可知生物試驗法為一種快速且能有效檢測出已知與未知類雌激素之方法，但複雜的環境水樣，則需再配合儀器分析方法以鑑定主要之類雌激素。

　　Many chemicals in the environment, in particular those with estrogenic activity, can disrupt endocrine signaling pathways. The complex mixture of compounds in the sewage discharged into river may contain xenoestrogenic endocrine-disrupting compounds, which can mimic the action of physiological estrogens on estrogen receptors (ERs) to cause reproductive interference and carcinogenesis in humans.
　　In this study, we detected estrogenic activity in Taiwanese rivers by using a yeast-based reporter gene assay combined with instrumental analysis. Samples were collected from five different rivers of Taiwan, including Danshuei, Yanshuei, Erren, Agnodian, and Gaoping River.
　　Estrogenic activity detected in the Erren River, especially in the tributary (Sanye River), was much higher than other rivers. Xenoestrogenic compounds in the Danshuei River were mainly originated from two tributary rivers (the Dahan and Keelung River), and relatively high estrogenic activity was also found in the midstream and downstream of the Yanshuei River and the Agondian River. Furthermore, estrogenic activity was also detected in the tributary rivers (the Wulo and Niuchou Creek) of the Gaoping River.
　　Xenoestrogenic compounds, including Bisphenol A, Nonylphenol, Estrone, Estriol, and Diethylstilbestrol were found in the Erren River by LC-MS/MS. From this study, bioassay is shown to be a rapid and efficient method to detect xenoestrogenic compounds, but it is necessary to combine with instrumental analysis to identify major xenoestrogenic compounds from environmental samples.

1. 內政部營建署，全國污水下水道用戶接管普及率及整體污水處理率統計表，2011
2. 凌永健，環境荷爾蒙的化學分析，環境檢驗雙月刊，2000
3. 行政院環境保護署，環境荷爾蒙管理計畫，2010
4. Bhatt RV: Environmental influence on reproductive health. International Journal of Gynecology & Obstetrics 2000, 70(1):69-75.
5. Oetken M, Bachmann J, Schulte-Oehlmann U, Oehlmann J: Evidence for Endocrine Disruption in Invertebrates. International Review of Cytology 2004, 236:1-44.
6. Sumpter JP: Endocrine Disrupters in the Aquatic Environment: An Overview. Acta hydrochimica et hydrobiologica 2005, 33(1):9-16.
7. Oh S-M, Park K, Chung K-H: Combination of in vitro bioassays encompassing different mechanisms to determine the endocrine-disrupting effects of river water. Science of The Total Environment 2006, 354(2-3):252-264.
8. Soto AM, Sonnenschein C, Chung KL, Fernandez MF, Olea N, Serrano FO: The E-SCREEN Assay as a Tool to Identify Estrogens: An Update on Estrogenic Environmental Pollutants. Environmental Health Perspectives 1995, 103(7):113-122.
9. Tapiero H, Nguyen Ba G, Tew KD: Estrogens and environmental estrogens. Biomedecine & Pharmacotherapy 2002, 56(1):36-44.
10. Mueller S: Xenoestrogens: mechanisms of action and detection methods. Analytical and Bioanalytical Chemistry 2004, 378(3):582-587.
11. de Vlaming V, Biales A, Riordan D, Markiewicz D, Holmes R, Otis P, Zander R, Lazorchak J: Screening California surface waters for estrogenic endocrine disrupting chemicals (EEDC) with a juvenile rainbow trout liver vitellogenin mRNA procedure. Science of The Total Environment 2007, 385(1-3):66-79.
12. Oh SM, Kim HR, Park HK, Choi K, Ryu J, Shin HS, Park J-S, Lee JS, Chung KH: Identification of estrogen-like effects and biologically active compounds in river water using bioassays and chemical analysis. Science of The Total Environment 2009, 407(21):5787-5794.
13. Blair IA: Analysis of estrogens in serum and plasma from postmenopausal women: Past present, and future. Steroids 2010, 75(4-5):297-306.
14. Roepke TA, Snyder MJ, Cherr GN: Estradiol and endocrine disrupting compounds adversely affect development of sea urchin embryos at environmentally relevant concentrations. Aquatic Toxicology 2005, 71(2):155-173.
15. Lombardi G, Zarrilli S, Colao A, Paesano L, Di Somma C, Rossi F, De Rosa M: Estrogens and health in males. Molecular and Cellular Endocrinology 2001, 178(1-2):51-55.
16. Diamanti-Kandarakis E, Bourguignon J-P, Giudice LC, Hauser R, Prins GS, Soto AM, Zoeller RT, Gore AC: Endocrine-Disrupting Chemicals: An Endocrine Society Scientific Statement. Endocrine Reviews 2009, 30(4):239-342.
17. Hilakivi-Clarke L, Cabanes A, Olivo S, Kerr L, Bouker KB, Clarke R: Do estrogens always increase breast cancer risk? The Journal of Steroid Biochemistry and Molecular Biology 2002, 80(2):163-174.
18. Clemons M, Goss P: Estrogen and the risk of breast cancer. N Engl J Med 2001, 334(4).
19. Deroo BJ, Korach KS: Estrogen receptors and human disease. The Journal of Clinical Investigation 2006, 116(3):561-570.
20. López de Alda MJ, Barceló D: Use of solid-phase extraction in various of its modalities for sample preparation in the determination of estrogens and progestogens in sediment and water. Journal of Chromatography A 2001, 938(1-2):145-153.
21. Newbold RR, Padilla-Banks E, Jefferson WN: Environmental estrogens and obesity. Molecular and Cellular Endocrinology 2009, 304(1-2):84-89.
22. Kuster M, López de Alda MJ, Hernando MD, Petrovic M, Martín-Alonso J, Barceló D: Analysis and occurrence of pharmaceuticals, estrogens, progestogens and polar pesticides in sewage treatment plant effluents, river water and drinking water in the Llobregat river basin (Barcelona, Spain). Journal of Hydrology 2008, 358(1-2):112-123.
23. Chen H-C, Kuo H-W, Ding W-H: Determination of estrogenic compounds in wastewater using liquid chromatography-tandem mass spectrometry with electrospray and atmospheric pressure photoionization following desalting extraction. Chemosphere 2009, 74(4):508-514.
24. Lin AY-C, Tsai Y-T: Occurrence of pharmaceuticals in Taiwan's surface waters: Impact of waste streams from hospitals and pharmaceutical production facilities. Science of The Total Environment 2009, 407(12):3793-3802.
25. Chen T-S, Chen T-C, Yeh K-JC, Chao H-R, Liaw E-T, Hsieh C-Y, Chen K-C, Hsieh L-T, Yeh Y-L: High estrogen concentrations in receiving river discharge from a concentrated livestock feedlot. Science of The Total Environment 2010, 408(16):3223-3230.
26. 吳建誼、丁望賢，環境荷爾蒙-壬基苯酚與雙酚A在臺灣水環境中之分析與流布調查，環境檢驗雙月刊，2000
27. 廖健森、張碧芬、袁紹英，環境荷爾蒙-塑膠添加物(鄰苯二甲酸酯類)之環境流布，環境檢驗雙月刊，2001
28. Duong CN, Ra JS, Cho J, Kim SD, Choi HK, Park J-H, Kim KW, Inam E, Kim SD: Estrogenic chemicals and estrogenicity in river waters of South Korea and seven Asian countries. Chemosphere 2010, 78(3):286-293.
29. Gray LE, Kelce WR, Wiese T, Tyl R, Gaido K, Cook J, Klinefelter G, Desaulniers D, Wilson E, Zacharewski T et al: Endocrine screening methods workshop report: Detection of estrogenic and androgenic hormonal and antihormonal activity for chemicals that act via receptor or steroidogenic enzyme mechanisms. Reproductive Toxicology, 11(5):719-750.
30. Kinnberg K, Miljostyrelsen D, Universitet S: Evaluation of in vitro assays for determination of estrogenic activity in the environment: Danish Ministry of the Environment, Danish Environmental Protection Agency; 2003.
31. 陳福安，環境荷爾蒙調查研究，行政院環境保護署環境檢驗所，2004
32. Routledge EJ, Sumpter JP: Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen. Environmental Toxicology and Chemistry 1996, 15(3):241-248.
33. Legler J, van den Brink CE, Brouwer A, Murk AJ, van der Saag PT, Vethaak AD, van der Burg B: Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line. Toxicological Sciences 1999, 48(1):55-66.
34. Pons M, Gagne D, Nicolas JC, Mehtali M: A new cellular model of response to estrogens: a bioluminescent test to characterize (anti) estrogen molecules. Biotechniques 1990, 9(4):450-459.
35. Mills LJ, Chichester C: Review of evidence: Are endocrine-disrupting chemicals in the aquatic environment impacting fish populations? Science of The Total Environment 2005, 343(1-3):1-34.
36. 黃壬瑰，內分泌干擾物質(環境荷爾蒙)生物檢測方法，環境檢驗雙月刊，2001
37. López de Alda MJ, Barceló D: Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water by fully automated on-line solid-phase extraction-liquid chromatography-diode array detection. Journal of Chromatography A 2001, 911(2):203-210.
38. Iwasaki S, Kato S, Takahashi M, Kimura T, Sakka K, Ohmiya K, Matsuda T, Matsui S: Analysis of estrogen-like compounds in the environment by high performance liquid chromatography bioassay. Journal of Bioscience and Bioengineering 2004, 97(3):216-218.
39. Rodriguez-Mozaz S, López de Alda MJ, Barceló D: Monitoring of estrogens, pesticides and bisphenol A in natural waters and drinking water treatment plants by solid-phase extraction-liquid chromatography-mass spectrometry. Journal of Chromatography A 2004, 1045(1-2):85-92.
40. Sodré FF, Pescara IC, Montagner CC, Jardim WF: Assessing selected estrogens and xenoestrogens in Brazilian surface waters by liquid chromatography-tandem mass spectrometry. Microchemical Journal 2010, 96(1):92-98.
41. Liscio C, Magi E, Di Carro M, Suter MJF, Vermeirssen ELM: Combining passive samplers and biomonitors to evaluate endocrine disrupting compounds in a wastewater treatment plant by LC/MS/MS and bioassay analyses. Environmental Pollution 2009, 157(10):2716-2721.
42. Lien G-W, Chen C-Y, Wang G-S: Comparison of electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization for determining estrogenic chemicals in water by liquid chromatography tandem mass spectrometry with chemical derivatizations. Journal of Chromatography A 2009, 1216(6):956-966.
43. Vega-Morales T, Sosa-Ferrera Z, Santana-Rodríguez JJ: Determination of alkylphenol polyethoxylates, bisphenol-A, 17[alpha]-ethynylestradiol and 17[beta]-estradiol and its metabolites in sewage samples by SPE and LC/MS/MS. Journal of Hazardous Materials 2010, 183(1-3):701-711.
44. Sanfilippo K, Pinto B, Colombini MP, Bartolucci U, Reali D: Determination of trace endocrine disruptors in ultrapure water for laboratory use by the yeast estrogen screen (YES) and chemical analysis (GC/MS). Journal of Chromatography B 2010, 878(15-16):1190-1194.
45. Saravanabhavan G, Helleur R, Hellou J: GC-MS/MS measurement of natural and synthetic estrogens in receiving waters and mussels close to a raw sewage ocean outfall. Chemosphere 2009, 76(8):1156-1162.
46. Grover DP, Zhang ZL, Readman JW, Zhou JL: A comparison of three analytical techniques for the measurement of steroidal estrogens in environmental water samples. Talanta 2009, 78(3):1204-1210.
47. 郭瀚文，以氣相及液相層析質譜儀分析具荷爾蒙效應物質之方法開發，國立中央大學，碩士論文，2004
48. Tso J, Aga DS: A systematic investigation to optimize simultaneous extraction and liquid chromatography tandem mass spectrometry analysis of estrogens and their conjugated metabolites in milk. Journal of Chromatography A 2010, 1217(29):4784-4795.
49. Granby K, Andersen JH, Christensen HB: Analysis of pesticides in fruit, vegetables and cereals using methanolic extraction and detection by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta 2004, 520(1-2):165-176.
50. 行政院環境保護署，淡水河流域污染整治管理計畫，2008
51. 經濟部水利署，水文年報歷年電子書，台灣水文年報，2009
52. 行政院環境保護署，99年度南部地區河川污染整治推動輔導及評析計畫，2010
53. 環保署環境檢驗所，河川、湖泊及水庫水質採樣通則，2005
54. 全國環境水質監測資訊網，http://wqshow.epa.gov.tw
55. 張晏禎、周佩欣，應用基因重組酵母菌評估河川流域中內分泌干擾物質之分佈，國立成功大學環境工程系，行政院國家科學委員會，2010
56. Fontanals N, Marcé RM, Borrull F: New materials in sorptive extraction techniques for polar compounds. Journal of Chromatography A 2007, 1152(1-2):14-31.