肌肉生長抑制基因（myostatin），其轉譯出之蛋白為肌肉生長抑制素，屬於TGP-β superfamily的一員。在肌肉發育上，肌肉生長抑制素扮演著負向調控的角色；若是此基因失去功能，則會造成肌肉備增的現象。另一方面，本實驗室已獲得石斑魚生長抑制基因的啟動子之全長為1936 bp，具有10個由CANNTG短序列組成的E-Box，可能為肌肉生長抑制啟動子相關的調節區。將全長以及系列剔除的肌肉生長抑制啟動子接到有螢火蟲螢光蛋白的質體，送進石斑魚鰭細胞株（GF-1）和活體內觀察其功能表現，找出肌肉生長抑制啟動子重要的調控區。之後更進一步利用區域性剔除、序列刪除和點突變之方法找出活化子和抑制子的確切位置，達到控制啟動子活性的目的。從結果得知，肌肉生長抑制啟動子最主重要的活性調控區是在E6及E5 E-Box區域，特別是E6區域的HNF-3b位置、E6 E-Box位置和E5區域的c-Ets位置。在之前的研究已得知神經壞死病毒和一些basic helix-loop-helix蛋白會影響肌肉生長抑制啟動子的活性，因此利用上述的調控因子，來觀察對肌肉生長抑制啟動子活性的影響，做為改變肌肉生長抑制啟動子活性的其中一種調控方式。最後發現Myo D、PKR、Poly[I:C] 皆可調控肌肉生長抑制啟動子的活性。

Myostatin, which belongs to TGF-ß superfamily, is the growth and differential factor-8. Myostatin is a negative regulator to restrict the growth of muscle. If Myostain does not work, we will find the penomenon of double-muscle.
On the other way, our lab have obtained the full length (1936bp) of grouper (Epinephelus coioides) mstatin promoter . Grouper mostatin promoter include 10 putative E-Boxes which are made up of CANNTG. We thought the E-Boxes maybe are the regulated region of muscle growth. Various constructs of the grouper Myostatin promoter were transfected into the grouper cells (GF-1 cell) and electroporate to juvenile groupers for analyze the promoter activity. Further, we observed the direct location of activator and repressor on grouper Myostatin promoter for regulating the activity. According to the experimental results, the most important regulation regions of grouper Myostatin promoter activity on E6 and E5 E-Box region, especially the HNF-3b and E6 E-Box position of E6 region, c-Ets position of E5 region.
By previous research, we have known nervous necrosis virus (NNV) and some basic helix-loop-helix proteins can affect the activity of grouper Mostatin promoter. Therefore, we use the reputational factors to observe the effect on grouper Mostatin promoter. We found that Myo D, PKR, Poly [I: C] could regulate the activity of grouper Myostatin promoter.

朱鴻鈞，全球漁業發展現況及未來趨勢分析-兼論台灣漁業發展現況，農業生技產業季刊，19：2-10 (2009)。

楊玉婷、陳葦芋、陳政忻,全球漁業發展現況及未來趨勢分析-兼論台灣漁業發展現況，農業生技產業季刊，20：24-29 (2009)。

魏志嶽，石斑魚肌肉生長抑制素之基因選殖與功能分析，國立成功大學生物科技研究所碩士論文，(2005)。

簡正修，點帶石斑魚肌肉倍增基因功能分析與其基因啟動子之調節，國立成功大學生物科技研究所碩士論文，(2007)。

陳宛兒，點帶石斑魚肌肉生長抑制基因之功能分析及分子調控，國立成功大學生物科技研究所碩士論文，(2009)。

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