在全球的疾病排名中，慢性腎臟病 (Chronic Kidney Disease, CKD)患者的成長極為快速，對於各個國家而言都是一個巨大的潛在性公共衛生問題。而CKD患者到了後期通常需要進行血液透析，對於國家的健康保險體制來說耗費龐大的資源，不僅造成資源分配不均問題，更是國家的一大負擔。會造成這樣的情況產生，是因為CKD初期並無明顯症狀，因此許多患者因無病識感而忽略其嚴重性，最終導致病情惡化。慢性腎臟病之併發症中，鈉離子佔據身體中大部分的陽離子，其失調所造成的風險最為常見的。因為它會造成病患昏迷、休克，若能縮短檢測時間就可以及早發現病情，透過調整飲食、盡早就醫保障病患健康。
在本研究中，使用”Sodium Green”此螢光感測器對鈉離子的專一性之螢光法，開發微流體晶片搭配微型化檢測平台，應用於鈉離子的濃度檢測。在晶片系統的試劑區填入試劑，藉由微流體晶片中的蛇形管進行混合，使其均勻反映後，由螢光強度值推導出鈉離子濃度。所提出的方法有效使用於鈉離子樣品檢測，濃度區間為10~200 mM。實驗結果顯示，使用所開發的微流體晶片系統檢測平台，鈉離子檢測的線性度R2近似為0.99，並且與國立成功大學醫院內科部腎臟科合作簽署IRB (Institutional Review Board)採集真實病患檢體進行檢測。微流體晶片系統檢測結果與成大醫院檢測數值做比較，兩者的相關係數為0.97。由此結果得出本文提出的系統為血液中鈉離子的檢測提供了低成本、製程簡單並且可靠的方法。

In the global disease rankings, the number of patients with Chronic Kidney Disease (CKD) continues to rise. This is because the patient lacks relevant knowledge of the disease and neglects the severity of the disease, which ultimately leads to the deterioration of the disease. In the complications of chronic kidney disease, sodium ions account for most of the cations in the body, and the risk caused by its imbalance is the most common. It may cause the patient to coma and shock. It is hoped that by shortening the testing time, the disease can be detected as soon as possible, and the medical treatment can be seen as soon as possible to protect the health of the patients.
In this study, the "Sodium Green" fluorescent sensor, which is specific to sodium ions, is used to develop a microfluidic chip with a miniaturized detection platform to detect the concentration of sodium ions.
The reagent and the sample are mixed through the serpentine tube in the microfluidic chip to completely react and fill the reagent area of the chip system, and then the sodium ion concentration is derived from the fluorescence intensity value. The proposed method is effectively used in the detection of sodium ion samples with a concentration range of 10~200 mM.
Experimental results show that using the developed microfluidic wafer system detection platform, the linearity R2 of sodium ion detection is about 0.99, and it cooperates with National Cheng Kung University Hospital to collect real patient specimens for blind testing. Comparing the test results of the microfluidic wafer system with those of Chengda Hospital, the correlation coefficient between the two is 0.97. From this result, the system proposed in this paper provides a low-cost, simple and reliable method for the detection of sodium ions in the blood.
Keywords: Chronic Kidney Disease, Microfluidic Chip, Sodium Ion, Fluorescence, Sodium Green

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