本論文目的藉由混合流動反應器（mixed flow reactor）探討微生物好氧降解甲苯之情形，並進一步探討環境因子（甲苯濃度、溶氧量及pH值）對反應器中甲苯分解菌社會結構的影響。混合連續流實驗依菌種來源不同分為兩階段進行，第一階段利用半連續培養泥漿法血清瓶中的懸浮菌液作為菌種來源;第二階段則是利用管柱試驗之出流液作為菌種來源，比較及觀察兩階段反應器內的環境因子與甲苯分解菌社會結構的關聯性。
在穩定操作條件下，兩階段甲苯混合連續流實驗甲苯生物降解效率高達 99%，甲苯生物降解的需氧量分別為 5.1 DO mole/Toluene mole 及 5.8 DO mole/Toluene mole。本論文並利用細菌分類學中的型態特徵描述分類法，以肉眼鑑定的方式，對出現在Nutrient Broth平板培養基上的菌株進行初步分類，在第一階段出現的菌株可分為九大類，第二階段亦分為九大類。比較兩階段反應器中環境因子對甲苯分解菌社會結構的影響發現，當反應器內溶氧含量高時，菌株NO.8為優勢菌種。而反應器內甲苯濃度高時，菌株NO.8消失，菌株NO.5為優勢菌種。

The aim of this study is to discuss the removal efficiency of toluene and the effect of environment factors（i.e., toluene concentration, dissolved oxygen and pH value） on toluene-utilizing bacteria community using mixed flow reactors. The experiments were conducted in two phases. In phase one, the suspended liquid from semicontinuous slurry microcosms was used as an inoculum in CSTR. In phase two, the effluent of column experiment was used as an inoculum in CSTR. The purpose of two phases is to find out the correlation between environment factors and toluene-utilizing bacteria community for each phase, and to compare with the correlation between phase one and phase two.

During steady-state, the toluene removal efficiency measured from both experiments was up to 99% and the oxygen consumption was determined as 5.1 DO mole/Toluene mole and 5.8 DO mole/Toluene mole for each experiment. According to Bergey’s Manual of Systematic Bacteriology, nine bacteria were isolated in phase one and nine in phase two. Compared with the bacteria community for both phases, bacterium NO.8 could survive when the dissolved oxygen was high. And when toluene concentration was high, bacterium NO.5 became the dominant bacteria in the reactor.

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