利用非侵入式量測人體皮膚膽紅素值廣泛用於篩選新生兒黃疸，本研究使用擴散傳播理論作為光子傳播理論的正向模型，利用線性Levenberg-Marquardt最小平方法作為反向模型計算出吸收及散射係數，我們使用漫反射光譜學(Diffuse Reflectance Spectroscopy，DRS)系統將量測到的漫反射光譜經由上述的理論模型計算轉換為組織的吸收係數(absorption coefficient,μ_a)與散射係數(scattering coefficient,μ_s)，再將吸收係數進行chromophore fitting，計算出新生兒皮膚中的膽紅素數值。
因抽血容易造成新生兒感染風險，且避免經過皮膚量測上易受膚色影響的問題，為了能即時得知嬰兒膽紅素數值，因此輕型化方便攜帶極為重要，本研究使用微型光譜儀(C12880MA)搭配400-600nm的LED光源，搭配DRS系統設計出一套非侵入手持式量測儀器，我們量測並計算得到新生兒皮膚的光學參數，在我們分析的光譜中，皮膚組織的主要色團為氧合血紅素(HbO_2)、血紅素(Hb)、直接膽紅素(Direct Bilirubin)、間接膽紅素(Indirect Bilirubin)與黑色素(Melanin)，我們能精準地量化出黑色素在光譜中的貢獻來排除經過皮膚量測易受膚色所干擾的影響。
本研究主要分為兩個部分，第一部分為利用仿人體假體來驗證手持式系統量測的可行性並與本實驗室精密完整量測系統做比較，結果顯示，即使考慮到強烈的黑色素干擾，也可以準確量化膽紅素濃度。第二部分為收取臨床上新生兒的漫反射光譜，主要量測新生兒身體上的二個部位(胸腔及腳底板)，在臨床研究中顯示，在不同部位測量的經皮膽紅素濃度與總血清膽紅素有顯著相關性，無論個體差異性，我們的手持式光學系統都是穩健可靠的。

In this study, we uses a miniature spectrometer (C12880MA), a 400-600nm LED light source and spatially resolved diffuse reflectance spectroscopy (SRDRS) to development a noninvasive handheld measuring instrument with the novel spectral analysis algorithm in order to measure and calculate the optical parameters of the neonatal skin. In the spectrum we analyzed, the main chromophores in human skin are oxyhemoglobin, hemoglobin, bilirubin and melanin. By taking melanin into consideration, This study will be conducted in two section of analysis. The first part is using the gelatin phantoms to verify the feasibility of the handheld system and compare it with the laboratory's complete measurement system. The results show that the bilirubin concentration can be accurately quantified even considering melanin interference. The second part, we measured 19 newborn neonates diffuse reflectance spectra including their sternum and the foots, and compare with total serum bilirubin (TSB) from blood tests.In clinical research shows that the concentration of handheld instrument measured at different sites was significantly correlated with total serum bilirubin,We measured and TSB values in both positions: sternum(r = 0.89) and foots(r = 0.79).So our handheld optical systems are robust and reliable

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