本研究建立在玻璃基板表面上形成平坦之胺基矽烷自組裝單分子層的製備程序，並利用原子力顯微鏡分析及靜態接觸角量測技術，針對所形成之自組裝單分子層的表面形態與潤濕性進行探討，以評估製程條件所造成的影響。
分析結果顯示，玻璃基板在經過所建立的基板清洗程序處理後，可將基板表面的粗糙度及水在基板上的接觸角，控制在非常理想的範圍內。而根據所建立的胺基矽烷自組裝單分子層製備程序，可在乾淨玻璃基板上形成平坦的自組裝單分子層。此外，雖然隨著改質基板保存時間的增加，自組裝單分子層的表面粗糙度並沒有明顯的改變，但透過靜態接觸角的量測，可發現基板上的自組裝單分子層在形成後，於大氣的環境下，自組裝單分子層中的分子排列位向仍會做某種程度的調整。
在此研究中也探討DNA分子在0.1 M 磷酸鹽緩衝溶液中於胺基矽烷自組裝單分子層上的吸附行為，並在大氣的環境下以原子力顯微鏡分析DNA吸附層的形態。分析結果顯示DNA是以直徑約80 ~ 100 nm、高度5 ~ 10 nm，以及少數直徑約200 ~ 400 nm、高度40 ~ 60 nm的吸附結構分布在基板表面上。此外，使用表面經胺基矽烷自組裝單分子層改質過的玻璃基板，可以得到分布較均勻的DNA吸附結構。

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The results demonstrated that after a glass substrate was treated with the established surface cleaning procedure, surface roughness of the substrate and contact angle of water on the substrate could be controlled within a reasonable range. With the established preparation procedure of an aminosilane SAM, a flat SAM could be obtained on a clean glass substrate. Moreover, though surface roughness of the SAM didn’t change significantly with time under the ambient condition, molecular orientation of the SAM seemed to adjust to a certain extent, according to the contact angle measurements.
Adsorption behavior of DNA molecules on the aminosilane SAM in a 0.1 M phosphate buffer solution was also investigated in this study, and morphology of the adsorbed DNA layer was analyzed by AFM under the ambient condition. It was found that DNA domains with 80 ~ 100 nm diameter and 5 ~ 10 nm height and a few domains with 200 ~ 400 nm diameter and 40 ~ 60 nm height were distributed on the substrate surface. Furthermore, a more homogeneous distribution of adsorbed DNA domains could be obtained with a glass substrate surface modified by an aminosilane SAM.

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