本研究為一新式的吸取式微流體聚合酶鏈鎖反應晶片，利用微機電系統的製程技術，分別製作了吸取式微流體晶片以及陣列式微型加熱器。晶片微流體晶片中包含三個微閥門及一位於中央之吸取式微流體傳輸薄膜，可即時傳輸之檢體及試劑於三個不同溫度反應區之間。此微流體晶片具有非常高的傳輸效率，在操作頻率為14.29 Hz及操作壓力為3 psi時，其傳輸速度高達18 μL/sec。另外，除了高效率的微流體傳輸晶片之外，本研究使用的微型加熱晶片為陣列式設計，可在邊緣補償其溫度變化，使反應區域內的溫度更加均勻，實驗結果顯示幾乎90 %以上的區域，溫度差異在±1℃之間，具有高度的溫度均勻性。本研究的另一個特點是開放式的反應腔體，其優點為易於在實驗之前作溫度的校正，且可直接從反應區域中將樣品放入或取出，不必另外設置樣品儲放槽，而增加製程上的困難；另外，此種設計亦有助於光學檢測。再者，控制模組可直接設定PCR反應次數及吸取式微幫浦的相關作動參數，在應用上將具有很大的彈性。
　　本研究利用國人肝病中常見的C型肝炎病毒(Hepatitis C Virus)作為生物檢測樣本，實驗結果顯示在此吸取式微流體PCR晶片上可成功地達成核酸增幅，並且在偵測極限上可達到5×103 copies/mL。

A new micromachined circulating polymerase chain reaction (PCR) chip is reported in this study. A new suction-type liquid transportation mechanism and three microvalves were used to comprise a new microfluidic control module to rapidly transport the DNA samples and PCR reagents around three bio-reactors operating at three different temperatures. When operating at a frequency of 14.29 Hz and a pressure of 3 psi, the sample flow rate of the microfluidic control module can be as high as 18 μL/sec. In addition, an array-type microheaters and open-type reaction chambers were adopted to improve the thermal uniformity in the reaction chambers and to facilitate the operation for temperature calibration. Experimental data from infrared images showed that the percentage of the uniformity area inside the reaction chamber with a thermal variation less than 1℃were over 90% for a denaturing temperature of 94℃. Three array-type heaters and temperature sensors were integrated into this new circulating PCR chip to modulate three specific operating temperatures for denaturation, annealing, and extension of nucleic acids. With this approach, the cycle numbers and reaction time of three reaction steps for a PCR procedure can be individually adjusted by the microfluidic control module. To verify the performance of this circulating PCR chip, a PCR process to amplify a detection gene (150 base pairs) associated with hepatitis C virus was performed. Experimental results showed that biosamples with concentrations ranging from 108 to 5×10^3 copies/mL can be successfully amplified by using this PCR chip. Therefore, the new circulating PCR chip can provide a useful platform for genetic identification and diagnosis.

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