在脊髓損傷的壓迫研究中，夾鉗壓迫因其易於建立而被廣泛使用。然而，先前的研究已經確定壓迫持續時間是影響脊髓損傷結果的關鍵因素，但尚未有統一的標準。儘管使用了統一製造的動脈瘤夾，但對其產生的閉合力或使用次數並沒有標準。在本研究中，我們描述了壓迫持續時間對夾鉗壓迫引起的SCI大鼠的嚴重程度的影響。我們從功能、組織學和電生理學角度評估了脊髓損傷的嚴重程度。此外我們還檢查了動脈瘤夾在反覆開合循環後的閉合力下降情況。我們通過在大鼠模型中應用80克動脈瘤夾在胸椎第10節處產生壓迫脊髓損傷，並測試了不同的壓迫持續時間（1、5、10、20和30秒），比較了它們的嚴重程度。對於最嚴重的脊髓損傷組，進行了胸椎第10節脊髓完全截斷。我們的研究通過功能、組織學和電生理學缺陷評估揭示了脊髓損傷嚴重程度與夾鉗壓迫持續時間之間的強正相關性。基於BBB評分，使用80克動脈瘤夾傷害的大鼠被歸類為重度脊髓損傷。即使是沒有持續壓迫，80克動脈瘤夾的快速夾擊也能導致中度至重度的脊髓損傷。體感誘發電位（SSEP）被證明是評估脊髓損傷嚴重程度的有效工具，也有利於幫助研究人員建立自身的脊髓損傷動物模型。我們發現動脈瘤夾的閉合力在五次開合循環後顯著下降，這表明應避免進一步重複使用。我們的研究結果為研究人員開發夾鉗壓迫誘導的脊髓損傷。

In the research of compression spinal cord injury (SCI), clip compression is widely used due to its ease of establishment. However, previous studies have identified compression duration as a critical factor affecting SCI outcomes, but no unified standard exists. Although manufactured aneurysm clips are used, there is no standard for closure force or the number of times they can be used. In this study, we evaluated the severity of spinal cord injury (SCI) in rats subjected to various clip compression durations. The severity of SCI was assessed from functional, histological, and electrophysiological perspectives. Additionally, we investigated the decline in closure force after the repetitive use of aneurysm clips. We induced T10 compression SCI by applying an 80-g aneurysm clip in our animal model, testing various compression durations (1, 5, 10, 20, and 30 seconds) and comparing their severity. For the most severe SCI group, a total transection over T10 was performed. Our study revealed a strong positive correlation between the severity of SCI—assessed through functional, histological, and electrophysiological deficits—and the duration of clip compression. Rats with SCI induced by an 80-g aneurysm clip were categorized as having severe SCI based on the BBB scale. Even a sudden impact without sustained compression resulted in moderate to severe SCI outcomes. The somatosensory evoked potential (SSEP) proved to be an effective tool for assessing the severity of SCI and for researchers establishing their own SCI animal models. We found that the closure force of aneurysm clips significantly decreased after five open-close cycles, indicating that further repetitive use should be avoided. Our results provide valuable reference points for researchers developing clip compression-induced SCI models in rats.

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