親手性結構擁有較明顯的旋光現象，使其在左右旋光波源下有極度不一樣的反應，利用了有限時域差分法(FDTD)之模擬方法，找出了在不同結構下對應旋光態的表現，也進行了一連串的模態分析。對於在奈米球微影術製程找出了較可行的結構。雙層旋轉C環金屬結構，利用了上下金屬的耦合，會造成能階分裂的現象發生，也因為上下層金屬C環結構之旋轉，造成有旋光性之現象發生，那當由自由空間轉換成較符合實際的情況時，兩層金屬中間有二氧化矽作為阻隔層，使其能階分裂下不明顯，造成上下耦合之效果減弱，導致在圓二色性之數值有明顯之減少，在製程方面必須要先填補下層金屬C環結構之開口，再蒸鍍出上層旋轉的C環結構，有製程上的技術困難需克服，所以在實驗上的結構選擇就為扭曲的金屬弧線結構(Twist-arc structures)。扭曲的金屬弧線結構(Twist-arc structures)，利用兩邊金屬弧線之耦合，使其在電場x、y偏振下之共振有紅、藍位移之現象產生，分別有低、高能階之模態差異，也因為左右兩邊金屬弧線有高度差異，使其在線性偏振下有相位延遲之現象，造成在左右旋光態峰值位移，展現出了較強的圓二色性之數值，接著也利用奈米球鏡微影術確實製程出了扭曲的金屬弧線結構，對於在實驗量測上之趨勢與模擬相符，當由空氣中量測轉換成在水中量測樣品，由於金屬結構上下所看到的折射率相近，導致基板對結構下的影響大大的減少，對於在圓二色性上的數值也有所提升。量測手性分子的選擇為半胱胺酸(cysteine)，由於在波長950(nm)、800(nm)下都有光學親手性增益(Optical Chirality Enhancement)之分布，在實驗量測上也確實量測出L-cysteine及D-cysteine造成的訊號差異，利用圓二色性總和(sum CD)之方法，也明顯找出對於不同手性分子下的訊號差異。

We fabricated of twist-arc structures using Nanospherical-Lens Lithography (NLL), and twist-arc structures are resonantly coupled, and the phase delay on the linear polarization is caused by the difference in the height of the metal arcs on both side, so the transmission of RCP and LCP are huge difference. This phenomenon is called the Circular Dichroism (CD), when the transmission of RCP and LCP are difference. Optical chirality enhancement (OCE) helps to distinguish regions with different handedness of the local field. For bio-sensing, it effectively resolves mirror-symmetric enantiomers. With Finite Difference Time Domain (FDTD) simulations, we can discover the signal of chiral molecule will be enhanced on the positive and negative structures. However, in experiment data, we did find that the signals of the two kinds of molecules are difference.

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