本實驗主要分成四個部分，首先是中孔氧化矽合成之性質研究，藉由氮氣等溫吸附脫附量測儀( N2 adsorption/desorption measurement ) 和高解析穿透式電子顯微鏡 ( High resolution transmission electro microscopy，HRTEM ) 探討孔洞結構和比表面積之情形。並利用3-氨丙基三甲氧基矽烷 ( 3-Aminopropyltrimethoxysilane，APTMS ) 進行氮官能基表面改質，增進金屬奈米顆粒均勻散佈性。接著在合成奈米載體的部分則是利用螯合劑以微波還原法的方式，將 Au、Pt、Ag、Cu 奈米顆粒負載在中孔氧化矽的表面，並以粉末 X 光繞射 ( X-ray diffraction，XRD ) 和 HRTEM 觀察奈米顆粒形貌、分散狀況和結晶結構。最後探討負載之金屬奈米顆粒吸附葡萄糖酵素，搭配電化學循環伏安法 ( Cyclic Voltammatry，CV ) 以偵測電流值與葡萄糖濃度變化之間關係，以評估此修飾電極之穩定性與重複性以及是否能運用在生物感測器上。
由氮氣等溫吸附脫附實驗顯示，合成出的中孔氧化矽具有高比表面積以及高孔洞體積。藉由表面胺官能基改質有效提升金屬奈米顆粒均勻性。利用微波還原法並搭配螯合劑的使用，由 TEM 結果顯示可以製備出粒徑小且散佈均勻之奈米顆粒。最後 CV 實驗結果顯示，負載金屬奈米顆粒能穩定葡萄糖酵素，保持酵素活性，用以偵測葡萄糖濃度變化，其中金奈米顆粒穩定性最佳，可作為生物感測器之載體用途。

This study reports the preparation and characterization of mesoporous silica supported metal nanoparticles and the electrocatalytic oxidation of glucose. the mesoporous silica was synthesized by the use of poly-(alkylene oxide) block copolymer. The high specific surface areas and large pore volume of mesoporous silica was observed from the N2 adsorption/desorption measurement. To distribute the metal nanoparticles uniformly on the surface of mesoporous silica, its surfaces were functionalized with amine group prior to addition of APTMS by post-synthesis. Then, the surface of mesoporous silica was supported metal nanoparticles, such as Au, Ag, Pt, and Cu, by chelating agent assisted with microwave synthesis. The surface morphology of mesoporous silica after microwave synthesis and the crystal structure of metal nanoparticles were both investigated by High Resolution Transmission Electron Microscopy (HRTEM) and X-ray Diffraction (XRD).
In order to show its application as a biosensor, the Glucose Oxidase (GOD) was adsorbed onto the matrix of the mesoporous silica-supported metal nanoparticles. Cyclic voltammetry measurement (CV) was employed to investigate the catalytic behavior of the mesoporous silica supported with different metal nanoparticles. The results showed that mesoporous silica supported metal nanoparticles could be used efficiently to detect the oxidation of glucose and the stability of the modified electrode. Our findings suggest that the mesoporous silica supported gold nanoparticles have a better stability than others. Therefore, the mesoporous silica supported gold nanoparticles can be developed as enzyme immobilization for biosensor construction.

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