本研究是用微機電製程技術來製作微電灑晶片( micro-electrospray chip)，改進微電灑晶片噴嘴切口角度進行電噴灑（electrospray）研究。傳統質譜儀使用的電灑噴嘴以不鏽鋼針管作為電噴灑噴射源，本研究利用微流晶片設計，因為微流晶片具有與電泳晶片整合的優點，目前發展中的許多蛋白質生醫晶片，在對於未知蛋白質檢測上大部分採用上述電泳晶片及微電灑晶片整合而成。因此，本研究針對微電灑晶片作實驗探討。
　　本研究利用顯微視流系統及操作電壓與電流來區分形成電噴灑的各個模態，並進一步找出電噴灑的最佳操作範圍。不同的切口角度，在錐噴流模態(cone-jet mode)的操作條件不同，角度較小要達到錐噴流模態的電壓較小，反之亦然。將各個切角的影響加以分析比較與歸納，並嘗試使用化學藥品(OTS)將晶片作表面改質，使晶片表面之接觸角提高以減少噴嘴口外濕潤面積，導致噴灑液滴減小。本研究最後用實驗方法驗證無因次化的 尺度法則，實驗結果近似此法則。

　　This study is using the micro fabrication technology to fabricate the micro-electrospray chip. In changing the nozzle angle of the micro-electrospray chip can become a research of electrospray. The traditional spectrum was using stainless tube to be the injector. This study uses the micro fabrication technology to fabricate the chip for experiment. The micro fabrication technology can integrate the experiment system on a chip. In the developing of biochip, they used to integrate electrophoresis and electrospray to detect the unknown protein. The thesis is trying to study the electrospray.

　　This study in using microscope-flow-visual-system to find out the optimum operation model in different combination of voltage and current. Different nozzle angle has different cone-jet mode. Smaller nozzle angle needs only smaller voltage. And bigger nozzle angle needs bigger voltage. This study use chemical treating to make surface changing in order to decrease the wetting area and also decrease the spray size. In the end of this thesis, the principle of the dimensionless factors published from the literature review have been proved by the results of this experiment.

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