本研究旨在了解奈米結構與表面電性對多巴胺偵測的影響。我們合成具羧酸、乙二醇及磷酸官能基團的數個聚(3,4)二氧乙烯噻吩對多巴胺進行偵測，從結果中顯示具備羧酸官能基的聚(3,4)二氧乙烯噻吩與多巴胺分子藉由靜電交互作用而得到較高的偵測界限。除此之外，我們亦評估電極在蛋白質吸附下的表現。將電極浸入牛血清蛋白、溶菌酶、纖維蛋白元溶液中6小時後並進行偵測，結果顯示蛋白的非特異性吸附降低了偵測靈敏度，然而該電極在1~50 μM多巴胺濃度中呈現相當一致的偵測能力，並能達到1 μM的偵測界限。這些顯示出電化學聚合官能基聚(3,4)二氧乙烯噻吩具備了輕薄穩定、蛋白質影響下的一致性，以及大量生產等特性。

In this research, we aim to understand the influence of nanostructures and surface charge on the detection of dopamine. We synthesize several functionalized poly(3,4-ethylenedioxythiophene) (PEDOT), including carboxylic acid, ethylene glycol, and phosphocholine functional groups. The results showed the nanostructured PEDOT with carboxylic acid groups provides best detection limit mainly contributed by electrostatic interaction between carboxylic acid and dopamine. Furthermore, we evaluate the performance of our electrodes in the presence of proteins. The electrodes are immersed into solutions containing BSA, lysozyme and fibrinogen for 6 hours before test. The results show the non-specific binding of protein lower the detection sensitivity. However the electrode presents a similar detection ability from 1 to 50 μM of domamine concentration, and achieves a lower detection limit about 1 μM. These show the electropolymerized functionalized PEDOT having the properties of light stability, consistency under protein influence and mass production.

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