在臨床上，骨肉癌的病患在經過手術治療後，多半會進行人工關節的置換手術，在配合術後的化療來抑制腫瘤復發，然而化療帶來強烈的副作用對於病人來說是很痛苦的負擔；利用磁性奈米粒子(MNPs)來進行物理性高溫療法(Hyperthermia)是目前最受注目的癌症治療方法，許多文獻顯示，當MNPs溫度提升到42~45 oC並持續加熱30分鐘後，能夠有效的殺死癌細胞，且能夠避免正常組織被大量的破壞，是具有癌症治療潛能之物理性療法。
氫氧基磷灰石(HA)為骨頭的主要成分，且具有良好的生物相容性與活性；此外，HA同時具備陽離子置換的特性，能夠使Ca2+與Fe2+離子進行置換形成具有磁性的magnetic-HA(mHA)奈米粒子，mHA粒子不僅具有HA的生物相容性與活性，同時也能夠與MNPs一樣被誘導加熱來進行熱治療；因此希望能夠將mHA奈米粒子被覆在植體表面，使人工植體不僅具備骨整合能力同時也能夠抑制骨腫瘤細胞再復發。
本實驗將利用共沉積方法合成mHA奈米粒子，並利用功能梯度生醫材料(FGBM)設計概念來將mHA被覆於鈦的表面，並且進行不同溫度與環境的熱處理，來了解熱處理條件對mHA的物化性質影響，最後利用體外的細胞實驗來探討FGBM被覆層對細胞的影響。
我們成功的利用共沉積的方法合成出具有生物磁性的HA奈米粒子，實驗結果證實，mHA不僅具有良好的細胞反應，同時也具備誘導加熱的特性；在真空熱處理實驗中，發現mHA燒結至900℃時擁有最佳之磁場強度，此外相較於未燒結之mHA，熱處理後的mHA在細胞的增生上也有較佳之反應；我們利用功能梯度生醫材料設計概念來將mHA被覆至鈦的表面，在抗壓實驗的結果顯示FGBM(383~402 MPa)相較於自然骨(~167 MPa)擁有更強的抗壓強度。
本實驗成功的製備出具生物活性與熱治療能力之陶瓷被覆層，經誘導加熱的實驗證實本被覆層能夠加熱至45~69℃；在未來骨癌病患手術後進行人工關節置換，本被覆層不僅能夠提供良好的骨整合性，並提供在未來腫瘤復發時的癌症熱治療能力，來取代化療對病患造成的負擔。

In clinical, the osteosarcoma patients would have artificial joint replacement after surgery and combined with chemotherapy to inhibit tumor recurrence. However, the side effect with the chemotherapy was too painful for patients. Hyperthermia by magnetic nanoparticles (MNPs) is one of many methods for cancer therapy. Previous studies indicated that when the MNPs temperature raised to 42-45oC and continued heating for 30 minutes, it could kill cancer cells and avoid disruption of normal bone tissue. Therefore, hyperthermia is a potential treatment of cancer therapy.

Hydroxyapatite (HA) had excellent biocompatibility and bioactive property, and it’s the main component of the bone. HA owned the cation-exchange capacity and the Fe2+ was used to substitute Ca2+. Thus the particles would possess not only the hyperthermia, but also the biocompatibility of HA. So we coated the magnetic-HA on implant, and expect it had the ability to promote osseointegration and the function of hyperthermia.

In this study, we synthesized mHA powders by the co-deposition method, and coated the mHA on the implant by using cold-pressed and sintered treatment, and let it become a FGBM(Functionally graded biomaterials), we also inverstigated the physical-chemical properties that underwent in different sintered temperature and environment. We also did the in vitro test to realize the cell responses to our FGMB.

We successfully prepared the biomagnetic-HA and the results show that mHA not only had a good cellular response, but also had the function of hyperthermia. After the vacuum heat treatment in 900 oC, the magnetic properties of mHA will be promoted and would have better cellular response than as-prepared mHA. The mHA coated prepare by the FGBM method, and the compression test results show that the compressive strength of all the specimens (383-402 MPa) were higher than natural bone (~167 MPa).

The coated mHA layer owned the good biocompatibility and the ability to hyperthermia. The induced heating experiment confirmed that mHA coated can heat up to 45-69 oC. In future, the patients after surgery could replace the mHA implant, which could promote osseointegration and owned the function of hyperthermia.

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