登革熱是一種新興的病毒感染,每年在世界各地增加。據世界衛生組織 (WHO) 稱,當登革熱病毒通過蚊子傳播時,病毒可在發病後4至5天內在血清、血漿、迴圈血細胞和其他組織中檢測到。因此,快速、靈敏、準確的登革熱感染診斷結果仍然是提供適當治療和管理的關鍵問題。本研究介紹了基於微流體二電泳(DEP)晶片的登革熱病毒(DENV)感染診斷。本研究使用的方法為流體動力學和DEP力,允許4G2塗層珠子和在設計晶片上標有螢光標記的DENV顆粒之間的免疫反應。利用DEP力捕獲微流體晶片中流動的修飾珠子(抗黃病毒單克隆抗體塗層珠子),用螢光標籤修飾的DENV可以通過免疫反應在改性珠子上捕獲。然後,通過ImageJ分析軟體對獲得的螢光信號進行量化。在DEP晶片上僅需要少量樣品(10-15 μL)來檢測DENV。該平臺可加速免疫反應時間,其中片上檢測時間小於3分鐘,並證明利用臨床登革熱病毒(DENV)樣本的DENV檢測能力低至102 pfu/mL。

Dengue is an emerging viral infection that is increasing annually worldwide. According to World Health Organization (WHO), when the dengue viruses are transferred by the mosquito-borne to humans, the virus can be detected in serum and plasma, circulating blood cells and other tissues for 4 - 5 days after the onset of febrile illness. Hence, a rapid, sensitive and accurate result of the dengue infection diagnosis is a crucial tool to provide proper treatment, prevention, and management to the disease. In this study, the diagnosis of dengue virus (DENV) infection based on microfluidic dielectrophoresis (DEP) chip was introduced. The method used in this study was hydrodynamic and DEP forces to allow the immuno-reaction between 4G2-coated beads and fluorescence-labeled DENV particles on the designed chip. The DEP force was employed to capture the modified beads (anti-flavivirus monoclonal antibody-coated beads) flowing in the microfluidic chip and the DENV modified with fluorescence label could be captured on the modified beads by immunoreaction. The obtained fluorescent signal was then quantified by ImageJ analysis software. A small amount of sample (10 - 15 μL) was needed for DENV detection on the DEP chip. The platform can be used to accelerate an immuno-reaction time, in which the on-chip detection time was less than 3 min, demonstrating an ability for DENV detection as low as 102 pfu/mL using the clinical dengue virus (DENV) sample.

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