本研究利用電性鑑別法應用於人類血色素(Hemoglobin, Hb)以及糖化血色素(Hemoglobin A1c, HbA1c)三明治免疫反應之檢測，結合阻抗式檢測電極晶片並利用金奈米粒子抗體標定技術，以助於偵測待測檢體之免疫反應並鑑別定量阻抗量測分析結果。實驗中利用電感、電容與自行開發的阻抗量測儀偵測阻抗晶片之電性訊號，藉由阻抗值改變分析判斷免疫反應變化，避免免疫色層分析法使用裸眼辨別造成之誤差，並建立三明治免疫分析法之電性量測系統。自行開發阻抗量測儀的過程中，為了穩定量測檢測試劑的阻抗電訊號，須先測試系統參數，例如檢測試劑反應時間以及檢測頻率等，達成人類血色素(Hb)與糖化血色素(HbA1c)的定量分析。本研究自行開發的阻抗量測儀其免疫檢測阻抗分析血色素(Hb)與糖化血色素(HbA1c)的濃度範圍分別為80~ 240 μg/mL以及2~ 10 %，且兩者的濃度檢量線決定係數(Coefficient of Determination)皆達0.9985以上，表示自行開發的阻抗分析儀可利用檢量分析線進行定量檢測分析，且在免疫檢測分析上提供一個創新的選擇。

This study, a novel electrode biochip for immunoassay was applied to detect sandwich-immunoassay of hemoglobin (Hb) and glycated haemoglobin (HbA1c), a gold nanoparticles (AuNPs) as a label of antibody to enhance immunoassay impedance quantitative analysis detection. Compared with traditional enzyme-linked immunosorbent assay (ELISA), we utilized the LCR meter instrument to detect the electric signal on the electrode biochip. By detecting the impedance changes because of sandwich-immunoassay reaction, a sandwich-immunoassay detection system was established. In the process of self-developed LCR meter, in order to stabilize the measurement of the impedance signal of the detection reagent, the system parameters, such as the reaction time of the detection reagent and the detection frequency, to achieve the quantitative analysis of hemoglobin (Hb) and glycated haemoglobin (HbA1c). In the study, the LCR meter has the immunoassay impedance analysis of hemoglobin (Hb) and glycosylated haemoglobin (HbA1c) in the range of 80-240 μg/mL and 2-10%, respectively, and the concentration of the two are determined by the calibration line. The coefficient is above 0.9985, which means that the quantitative analysis line can be used for quantitative detection and analysis, and provides an innovative option in immunoassay analysis.

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