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研究生: 鍾劍鋒
Chung, Chien-Feng
論文名稱: 設計與模擬微量樣本聚焦/分離/切換收集在電驅動微流體系統晶片之應用
Design and Numerical Simulation of Electrokinetically Driven Sample Flow with Focusing, Dispensing and Collection in a Microfluidic System
指導教授: 楊瑞珍
Yang, Ruey-Jen
學位類別: 碩士
Master
系所名稱: 工學院 - 工程科學系
Department of Engineering Science
論文出版年: 2004
畢業學年度: 92
語文別: 中文
論文頁數: 71
中文關鍵詞: 微流體系統微機電數值模擬電滲流
外文關鍵詞: numerical simulation, mems, microfluidic system, electroosmotic flow
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  •   本文主要是在電滲流場的理論基礎下,設計一種微流體系統晶片應用於生物晶片系統中的檢測技術,以數值模擬的方式模擬電滲流場在微流體系統的應用,其應用在微量樣本的截取及分配。
      模擬電滲流場所使用物理模式包括(i)描述電雙層分布之Poisson-Boltzmann方程式(ii)描述外加電場電位勢分布之Laplace方程式(iii)描述電滲流流場之包含電驅動力的Navier-Stokes方程式。而主要研究重點分為兩項,茲說明如下:
    (一)、針對電滲流場在微流體晶片系統中應用於樣本的聚焦、切換、收集所造成進料、截取、分類的設計問題進行探討。
    (二)、針對電滲流場中控制電場的設計及現象影響作一綜合的討論及解決方式的研究。
      本文的結果可以得知在微流體系統晶片上的應用是可行的,可以提供實作上的參數設計及樣本傳輸過程中可能發生的問題改良,最後希望能藉由微機電製造技術的方式發展自動樣本檢測晶片,其產品可以大幅降低生化檢測分析的成本及效率,讓微系統科學能有效的運用在工程問題改善。

      This study presents a novel design delivery finite amount of sample by eletrokinetically-driven flow transport process. The novel design is numerically simulated by the following physical models: (i) the Poisson-Boltzmann equation for electrical double layer (EDL) potential, (ii) the Laplace equation for the externally applied electrostatic field, and (iii) the Navier-Stokes equations modified to account for the electro-kinetic body force.     This research consists two main parts; first we present a systematic, the sample approach for focusing and dispensing on a microfluidic system. Second, we present the voltage control on this system, which is used to influence the sample plug leakage and diffusive phenomena during the sample transport process.
      The designed microfluidic system can be used to achieve on chip capillary electrophoresis applications. The numerical simulation techniques developed in this work provide a useful tool for parametric studies. We believe that the proposed microfluidic system is a useful micro-total-analysis system and can be applied to a clinical hematology test.

    中文摘要 I 英文摘要 II 致謝 III 目錄 VI 圖目錄 VII 符號說明 IX 第一章、序論 1 1-1、前言 1 1-2、生物晶片系統 2 1-3、微流體理論 4 1-4、電雙層的形成機制 6 1-5、電滲流理論 7 1-6、研究動機 9 1-7、本文架構 11 第二章、電滲流流場之統御方程式 12 2-1、序論 12 2-2、基本假設 13 2-3、描述電雙層分布之Poisson-Boltzmann方程式 13 2-4、描述外加電場電位勢分布之Laplace方程式 16 2-5、描述電滲流流場之Navier-Stokes方程式 18 2-6、描述檢測液(染液)濃度分布之濃度方程式 23 第三章、微流體系統晶片設計 26 3-1、晶片設計概念 26 3-2、光罩底片之繪製 26 3-3、檢測液的持續進樣方式及電路系統 27 3-4、檢測液樣本分離收集方式及電路系統 28 3-5、模擬尺寸與環境參數的設定 28 3-6、結論 30 第四章、結果與討論 31 4-1、序論 31 4-2、預集中效果所得到的帶寬影響 31 4-3、樣本的切換收集機制 32 4-4、幾何形狀與電場所影響洩漏現象的比較與討論 33 4-5、微量樣本在傳輸過程的分佈 34 4-6、管道中的廢液清除 36 第五章、總結 37 5-1、結論 37 5-2、未來展望 38 參考文獻 39 圖表 42 附錄A 55 自述 58

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