在過去的研究中，實驗室的黃世昱學長嘗試使用o-ABDI的衍生物與銅(II)錯合物進行養晶的過程中得到環化的產物，在過去的文獻當中也有使用銅離子做為添加物所進行的含氮雜環合成反應，這展現了銅(II)作為添加物進行環化的可能性。
在本文中將研究以綠色螢光蛋白衍生物o-ABDI為起始物進行親核取代改變其鄰位上的取代基，再與銅(II)進行環化反應並觀察含有不同取代基對於反應的影響，還有產物的光譜性質差異。
我們使用o-ABDI以及其鄰位取代基改變為帶有立體障礙、酸鹼性或拉電子基的取代基進行環化反應，發現環化後的產物在吸收光譜上與過去實驗室將具有diol moiety的化合物進行環化後的光譜有相似的結果，都產生藍移的現象，並且這樣的現象也出現在螢光光譜上。

In the past research, Shih-Yu Huang, a senior in the laboratory, used o-ABDI derivatives and copper(II) complexes to obtain cyclized products, instead of expecting crystal product. In the past literature, there are also nitrogen-containing heterocycle synthesis reactions using copper ions as additives, which shows the possibility of copper(II) as additives for cyclization.
In this paper, we try to change the substituent on the ortho-position of o-ABDI derivatives and observe the effects of different substituents on the cyclization reaction and try to figure out the influence of o-ABDI derivatives with different substituents and the different in the spectral properties of the products.

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