細胞藉由黏著分子能夠與細胞和細胞外基質連結。黏著行為會受到細胞內部生理調控與外在環境的影響，對於細胞型態、移動、
運動和分化等生理方面及癌細胞轉移等病理方面皆扮演重要角色，
因此細胞的附著能力也意味著細胞執行正常生理行為的表現。目前有許多技術可用於量測細胞的附著力，如微吸管、光學鑷等，其中原子力顯微鏡有易於調控參數、生理環境下量測、高解析度等優勢，搭配使用細胞取代針尖功能的細胞探針技術，可量測細胞與基材、細胞與細胞之間的細胞附著力。基材的軟硬度被認為會影響細胞的行為，每種細胞對於軟硬度的感知與反應也不盡相同。
本研究欲探討短時間內基材軟硬度與細胞附著力的關係。實驗選用纖維母細胞，使用原子力顯微鏡搭配細胞探針技術去定量細胞附著力，細胞與基材的接觸時間定於30秒、60秒、90秒及300秒，所有基材表面塗佈有膠原蛋白第一型，硬度分成1037 Pa、10930 Pa及玻璃基材(控制組)，並且比較細胞附著力與接觸時間、基材硬度的關係。統計分析方法採用單因子變異數分析和涂基HSD檢定，
定義p<0.05有顯著差異。
實驗結果顯示在相同的接觸時間下，隨著基材硬度增加，細胞附著力有逐漸上升的趨勢，細胞附著力大小在1037 Pa、10930 Pa和玻璃基材，接觸時間30秒分別為296.99±107.73、459.69±228.51、
620.01 pN，接觸時間60秒分別為344.96±198.85、561.84±283.58、1917.3±344.28 pN，接觸時間90秒為347.44±125.22、644.75±358.57、2519.76±685.06 pN，接觸時間300秒分別為482.11±194.34、1820.11±949.29、3373.45±1867.02 pN，統計分析顯示三種基材硬度在各組的接觸時間的細胞附著力互相都有顯著差異。
另外，觀察三種硬度基材的細胞附著力與隨著時間增長的趨勢發現硬度1037 Pa的細胞附著力上升不明顯，硬度10930 Pa的細胞附著力在90秒到300秒之間有顯著上升，玻璃基材的細胞附著力在30秒至300秒之間呈現明顯上升且趨向穩定平衡的趨勢。
從結果發現細胞會受到基材軟硬度的影響而有不同表現，未來可以結合生化分析與螢光顯微鏡等技術，對於細胞內部黏著分子的分佈與變化有更多了解。

The cell can link another cell and ECM by adhesion molecules. The behavior of cell adhesion is influenced by the cell physiology and external environment. It plays an important role in cell morphology, motility, movement and differentiation, and cancer metastasis etc. So the ability to cell adhesion means that the cell carries out the normal physiological behavior. At present, a lot of technology can be used to quantify cell detaching force, for instance, micropipette, optical tweezers and so on. AFM has a lot of advantages, like parameters control easily, high resolution, and measuring in air. AFM combined with cell probe replacing tip function could measure the cell detaching force between cell-substrate and cell-cell. The rigidity of the substrates is considered to affect cell behavior. The rigidity sensitivity and respond of each cell is not the same.
The poupose of this study is to investigate the relation between the rigidity of substrate and cell detaching force. Fibroblast cell was used to quantify cell detaching force. The contact times were set at 30, 60, 90, and 300 seconds. All the surfaces of substrates were coated with collagen type Ⅰ. The rigidity of the substrates were 1037 Pa, 10930 Pa and glass (control group). Statistics is by one-way ANOVA and Tukey HSD.
Under the same contact time, the detaching force rise gradually with increasing substrate rigidity. Cell detaching force on 1037 Pa, 10930 Pa and glass substrates were 296.99±107.73, 459.69±228.51, and 620.01±211.98 pN respectively for 30 seconds contact time; the forces were 344.96±198.85, 561.84±283.58, and 1917.3±344.2 pN for 60 seconds; the forces were 347.44±125.22, 644.75±358.57, and 2519.76±685.06 pN for 90 seconds.; and the forces were 482.11±194.34, 1820.11±949.29, and 3373.45±1867.02 pN for 300 seconds. The cell detaching force among three rigidity substrates were significant different on each contact time.
The detaching force increased slowly on 1037 Pa, increasing notablely from 90 to 300 seconds on 10930 Pa, and increasing notablely to be stable from 30 to 300 seconds on glass.
Our results showed cell adhesion could be influenced by substrate rigidity. This cell probe technique can be further used for parameter study on cell substrate interaction in the future.

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