全球缺水的問題日益嚴重，已然成為一項迫切的全球性挑戰。這不僅影響人類的生存和發展，也對全球安全和經濟造成潛在威脅。缺水的原因涵蓋許多面向，包括氣候變化、人口增長、不合理的用水管理、水污染，以及貧富差距導致的資源分配不均等因素。其中，廢水回用是一個增加人類可使用之淡水資源的途徑。為了解決水資源不足的問題，世界各國積極發展研究再生水的處理技術，並建立許多再生水廠，期能將生活污水等廢水淨化至能夠再利用的水質。
為探討再生水中具內分泌干擾活性之新興污染物種類，本研究以報導基因重組酵母菌試驗法檢測再生水廠中各處理單元之出流水，並藉由效果導向分析，以層析方法分離樣本後，再次進行生物試驗以確認具有內分泌干擾活性之集份，後續透過非目標分析嘗試確定集份樣本中之化合物。
內分泌干擾活性試驗結果顯示，於所有樣本中可測得抗鹽皮質激素活性 (3.5 - 75.1 μg Spironolactone equivalent (SPL-EQ)/L)，以及類芳香烴活性 (6.3 - 59.0 ng β-Naphthoflavone equivalent (β-NF-EQ)/L)。抗雄激素、類甲狀腺激素或抗甲狀腺激素活性可於進流或部分單元出流水中測得，但並未出現於放流水中。於效果導向非目標分析的結果顯示，於各再生水廠之進流水中識別出藥物合成原料如3,5-Dichlorobenzoic acid、Octadecanoic acid, 2,3-Dihydroxypropyl ester、2-Keto-L-gulonic acid、2,4-Dichlorobenzoic acid，抗氧化劑如Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-，以及其他化合物如Tridecanoic acid. 12-methyl-, methyl ester、Methyl Octanoate、2-Azido-2,4,4,6,6,-pentamethylheptane等。於放流水樣本中，大部分物質皆被去除，展示出再生水廠處理單元之去除能力。

With the advancement of industry and treatment technologies, the application of reclaimed water has become increasingly widespread in many countries. Reclaimed water can be used for agricultural irrigation, groundwater recharge, and even as ultrapure water for industrial processes. However, the presence of unknown contaminants in reclaimed water may not only affect product yield but also pose potential environmental hazards. Therefore, identifying the types of contaminants present in reclaimed water is of critical importance.
Results showed anti-mineralocorticoid activity (3.5 - 75.1 μg Spironolactone equivalent (SPL-EQ)/L) and aryl hydrocarbon-like activity (6.3 - 59.0 ng β-Naphthoflavone equivalent (β-NF-EQ)/L) in all samples, while no significant androgenic or mineralocorticoid-like activities were observed. Anti-androgenic activity (66.1 - 80.9 μg Flutamide equivalent (FLU-EQ)/L) was found in influents, with thyroid hormone-like (0.06 - 0.14 μg Triiodothyronine equivalent (T3-EQ)/L) and anti-thyroid activities (51.0 - 66.6 μg 3,5-Di-tert-butyl-4-hydroxybenzoic acid equivalent (BHT-COOH -EQ)/L) detected at certain sites. EDA revealed pharmaceutical intermediates, antioxidants, and fatty acid derivatives in influent samples. Most compounds were effectively removed in effluents, highlighting the treatment effect of reclamation processes.

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