牙科黏著的目標是希望能形成黏著劑與牙齒之間長期緊密穩定的介面，維持邊緣密封以及臨床的耐久性，然而現今的牙科材料所形成的黏著劑混和層(hybrid layer)介面會隨著時間降解而導致樹脂-牙本質黏著介面失敗。仿生再礦化是保護黏著介面完整的一個可能的方法, 旨在模仿自然的礦化過程。磷灰石以微晶形式在膠原蛋白網絡間沉積，可再礦化修復有缺陷的樹脂-牙本質混合層，增強黏著介面耐久性。
最近市面上出現的通用型黏著劑可使用酸蝕沖洗或自酸蝕模式黏著齒質，主要是內含酸性樹脂單體10-甲基丙烯酰氧基癸基二氫磷酸酯（10-MDP），其磷酸鹽基團可鍵結到羥基磷灰石上，形成特殊的分層MDP-Ca結構。本研究的目的是檢視通用型黏著劑所含的10-MDP對於混合層仿生再礦化的可能影響，以及對提升黏著強度、防止黏著介面的降解之效應。
在實驗中，首先以X射線光電子能譜來檢查黏著劑塗層的表面經再礦化溶液浸泡後元素組成的改變。結果顯示，三種通用型黏著劑塗層在再礦化溶液中儲存一個月後，表面鈣含量以CLEARFIL S3 Bond Universal (CSU)黏著劑最高。再以薄膜X光繞射儀檢查黏著劑塗層經再礦化溶液浸泡後結晶的改變。結果顯示，三種通用型黏著劑塗層在再礦化溶液中儲存一個月後，以CSU黏著劑有最明確的氫氧基磷灰石節結晶表現。以銀染的方式檢測浸泡模擬體液和仿生再礦化溶液的通用型黏著劑樹脂-牙本質黏著樣品介面微滲漏的表現，結果在三個月和六個月儲存期後，所有組別的介面都沒有明顯增加微滲漏。另外，樹脂-牙本質黏著樣品在初始及分別儲存在模擬體液與仿生再礦化溶液3個月後，以微拉伸黏著強度測試，並以電子顯微鏡檢查微拉伸斷裂模式的分佈與黏著介面的微觀型態，結果顯示仿生再礦化溶液相較一般模擬體液可促進再礦化的發。由以上結果，所有通用型黏著劑配合酸蝕步驟相較自酸蝕提升黏著強度，其中配合酸蝕沖洗的CSU組別強度測試表現最佳，自酸蝕模式的All-Bond Universal (ABU)組表現最差。

The goal of adhesive procedures is to form and maintain a tight adhesive-dentin interface, marginal sealing, and the stability. Regardless of the advances in dental materials, the hybrid layer in adhesive interfaces still degrades over time. Biomimetic remineralization of the hybrid layer has been proposed as a salvaging strategy, as the deposition of apatite on the denuded collagen network may repair defective resin–dentin interfaces and increased durability.
The recently introduced multi-mode universal adhesives is designed to bond to tooth via the etch-and-rinse or self-etching modes, mostly rely on the acidic resin monomer 10-methacryloyloxy-decyl dihydrogen phosphate (10-MDP). The functional monomer bonds through its phosphate groups to hydroxyapatite and particularly forms a regularly layered MDP-Ca structure. Therefore, this study was aimed to examine the ability of MDP-based universal adhesives in remineralizing hybrid layers, and thus to enhance the bonding and prevent degradation.
In the study, x-ray photoelectron spectroscopy (XPS) was used to examine the elemental composition on the surface of adhesive coatings after soaking in the biomimetic remineralization medium (BRM). The results showed more calcium contents in CLEARFIL S3 Bond Universal (CSU) adhesive after 1-month BRM storage. X-ray diffractometer (XRD) was used to check the surface crystallization of the adhesive coating after immersion in the BRM. Only CSU adhesive showed characteristic peaks at 2θ= 26°, 29°, 32° after 1-month BRM storage. In addition, a nanoleakage test by silver staining did not show significantly increased nanoleakage in interfaces of universal adhesives after 3- or 6-month SBF/BRM storage. Finally, resin–dentin bonding was examined by a microtensile bond strength test immediately after bonding and after 3-month storage in either SBF/BRM. The distribution of fracture modes and microscopic morphology of the adhesive interface were examined by SEM. The results showed that there were remineralizations in all groups after 3-month BRM storage. In conclusion, three universal adhesive used in etch-and-rinse mode are more effectively to enhance bond strength compared to those in self-etch mode. CSU group with etch-and-rinse mode showed the highest bond strength, and the ABU group with the self-etching mode the lowest performance.

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