膜片箝制(Patch Clamp)技術，是電生理學中研究離子通道機制最成熟完備的一項工具，傳統膜片箝制方式是使用玻璃吸管(pipette)移動到要紀錄的細胞上。此方式可得到正確且精準的資訊，但卻需要純熟的操作技術，且相當耗費時間導致只能得到少量的紀錄資訊。電壓箝制(voltage clamp)則是一種量測離子通道性質的方式，給予一定程度的電壓來記錄細胞膜間的離子電流進出以及反應，藉此可觀測細胞的動作電位(action potential)變化的情形，從而估算通道電容或電阻的參數。
此研究延續了本實驗室長久以來的成果，除了將用以取代傳統玻璃電極的新式膜片鉗晶片製造出來，並對其效能加以改善，提升製程良率以及減低製造成本，本研究中利用改變晶片的結構材料以及製造程序，再加上利用BOE潤飾微孔洞之製程，把此膜片鉗晶片之微孔洞能縮小到1μm以下的程度，將能有效提高量測離子電流的準確性，而整體的結構層厚度能相當一致地縮減到7μm以下，理論上，局部串聯電阻將可降低，其值也較穩定。另外，製造上取消了需要昂貴專利的BOSCH ICP製程，並且以仿製面型微加工的方式取代了精準度較差的體型微加工，在大量提高量率後製造成本也隨之降低，使此晶片將來在學術以及市場上更具競爭潛力。

Patch clamp is a well-developed electrophysiological recording technique used to study ion channel function and regulation. The conventional method of performing patch clamp technique employs a glass micropipette onto the cell by manual manipulation. Despite this technique is extremely sensitive and information-rich, but requires a highly-skilled operator and is limited in throughput. Voltage clamp that determine the behavior of the ion channel conductance responsible for the generation of the action potential is the another method to record the flow of ionic current across the cell membrane. The method is held a constant membrane potential while the ionic current flowing through the membrane is measured.
　This research continue the achievement of our laboratory. We not only replace traditional patch clamp of glass electrode by patch clamp chip, but also improve its efficacy, field and cost down. In this research we reduce the patch hole below 1μm using the method of changing structure material, process flow, and an additional process of BOE dipping. Then the accurate rate could be arised. By this we also reduce the whole structure thickness of the chip identically which can make local series resistance lower and stablely. Besides in the process we cancel the BOSCH ICP that has expensive patent cost, and replace bulk micromachining process by surface micromachining like process to raise filed, hence this chip would have much more competition potential.

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