本篇論文主要目的在於改善以Rhodamine為基底的化學感測器之水溶性，並且使其可完全溶於水相，如此一來在感測重金屬離子過程中，便不需要加入任何有機溶劑來幫助溶解，使得系統更加單純，這樣便可忽略添加之有機溶劑是否對分析物有影響等問題。
在提高水溶性而又不失原先的感測條件下，藉由導入β-環糊精，利用其桶狀結構且內部為疏水性質，與Rhodamine結構中末端的N,N-diethyl group以疏水-疏水作用力結合，利用非共價鍵的形式，藉由相互作用力而形成包容錯合物，藉此提高Rhodamine-based感測器於水相下之溶解度。
本研究合成一以Rhodamine B為基底之螢光感測分子RB，並與β-環糊精在水相下混合形成RB-CD包容錯合物，可有效感測Pd2+離子且具有良好的選擇性；與鈀離子螯合後會造成RB-CD結構中的spirolactam ring開環，共軛鍊段拉長，導致光學性質有急遽的改變，例如在560 nm可見光區之吸收度大幅提升，使得溶液呈現深紅色；而螢光部分則是在540 nm所放出的綠色有大幅的銳減，且放光位置則位移至600 nm轉變成微弱的橘紅色；此螢光淬熄的主因來自於開環後的RB-CD即便在低濃度條件下，大多還是以二聚體的形態存在於水相下，而二聚體的濃度又與量子產率成反比，又藉由Job’s plot 以及質譜儀可得到RB-CD與Pd2+的配位比例是2:1，這正好又加強了二聚體形成的機會，使得螢光強度隨著Pd2+濃度上升而逐漸下降。
將化合物RB溶在水:乙醇 (體積比1:1) 條件下，發現其感測Pd2+的效果與本研究RB-CD系統相同，由此可證明導入β-環糊精不僅可提高水溶性，且並不會影響化合物RB原有的感測性質。

To improve water solubility of Rhodamine-based sensor, water soluble inclusion complex RB-CD was synthesized by host-guest interaction between RB and β-cyclodextrin. RB-CD shows chemosensing property and good selectivity for palladium ions (Pd2+). Addition of Pd2+ into RB-CD changes optical properties from colorless to red due to spirolactam ring opening. Moreover, fluorescence self-quenching is triggered by dimeric product RB-Pd2+-RB. It was concluded that threading of β-cyclodextrin onto RB does not affect the sensing property but promote the solubility of RB in water.

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