在農作物的生長過程中，經常遇到許多壓力逆境，其中生物性的病害造成的農業問題一直以來也是農業災害中的重大問題之一。科學家自從綠色革命以來就以化學農藥抑制植物病原菌的擴散，但近年來也逐漸發現有抗藥性的菌株誕生，顯然化學農藥並不是長久之計，並且化學農藥還有可能負面影響土壤生物多樣性。邁入21世紀，人們逐漸反思該如何與環境共存，最近在許多國家和地區透過改善農業上的法規以及經由科學上的研究努力實踐永續農業的理想。過去本實驗室透過總體基因體學分析長期的農業試驗田，在分析中顯示具有豐富的抑制病原菌的菌株在其土壤中，本篇的研究透過試驗田土壤的採樣挑選出四株具有抑制病原菌能力的菌株，並分別測試了不同溫度、鹽分、酸鹼值對生長的影響了解篩選菌株的最適生長環境。透過定量菌液，此四株菌分別在三種不同培養基與三種在台灣常見的植物病原性真菌(立枯絲核菌、稻熱病菌、徒長病菌¬)進行對峙培養。結果表明代號R1OF 821的菌株對於三種病原菌在不同環境下都擁有良好的抑制效果。接著，我們想要更進一步的探討混合菌株可否產生超過於單一菌株的抑制能力，因此我們將有抑制效果的菌株做混和並進行實驗，結果並沒有超出於原本的單一菌株。透過一系列的實驗，我們進一步的驗證了長期有機試驗田篩選出的菌株具有對抗植物病原菌的能力。未來希望能再進一步的利用盆栽試驗以及田間試驗證明此篩選菌株有助植物抵抗常見的農業病原菌，對永續農業有實質性的貢獻。

Regarding the Green Revolution, although it led to a significant increase in yield in the past, the use of chemical pesticides also caused considerable damage to the land. To improve the land and transition towards sustainable agriculture while promoting sustainable development, the development of biological agents has become a prominent research focus for institutions worldwide. In this study, four strains were isolated from soil in paddy-upland rotation fields where organic fertilizers were applied. These selected strains demonstrated inhibitory effects against three phytopathogens: Rhizoctonia solani, Fusarium moniliforme, and Pyricularia oryzae. Observations on the culture medium revealed distinct fungal hyphae growth patterns near the selected strains compared to their normal growth state in other areas. This suggests that the selected strains might secrete secondary metabolites that inhibit fungal growth. Additionally, certain strains (R1OF 821 and R1OF 822) displayed bacterial swarming behavior, indicating their motility and a significant advantage in competing against phytopathogens. In conclusion, these selected strains exhibited significant resistance to the phytopathogens. We hope that the selected strains can also display excellent antimicrobial effects in both pot and field trials, thus enabling their application in sustainable agriculture.

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