本研究是利用分子模版技術，萃取環境樣品中多環芳香族化合物(PAHs)，並且利用分子模版之特性，更有效的萃取出PAHs中之有毒物質Benzo[a]pyrene (BaP)，研究中透過各種材料測試，以及採集市售香煙主流煙進行分析，所採集之樣品由氣相層析質譜儀(GC/MS)進行分析。
分子模版主要是經由官能基單體與交聯劑之間的作用，對於目標吸附物質形成特殊孔洞，以達到選擇性的萃取之目的。本研究室自行研發之分子模版，以結構相似之7-methylbenzo[a]pyrene(7-MeBP)作為目標分子製備模版，透過分子模版的高選擇性，有效的吸附目標汙染物，且與傳統萃取技術比較，不僅可以減少時間、人力，使用溶劑也較少。
本實驗依照不同官能基單體與交聯劑，配置三種不同配比之分子模版，在目標分子的移除之後，分為控制組及對照組，進行各種材料測試，如:吸附量測試、選擇性測試、回收率測試、環境樣品測試、掃描式電子顯微鏡(SEM)等，實驗結果顯示，MIP-1為效果最佳之分子模版，其吸附容量為20.78 ± 1.7 (μg/g)，針對BaP的選擇性與回收率皆為90 %以上，而在環境樣品測試部份，分為Mild Seven sky blue(七星淡菸)及Marlboro light(萬寶路淡菸)兩種香菸之主流煙測試，分子模版所吸附之BaP量，為濾紙採樣吸附之80 %。由各項結果顯示，分子模版可以有效的吸附16 種PAHs中之BaP分子，且可降低儀器分析背景值之干擾，提高訊號解析度。此外與傳統萃取方式相較，分子模版運用於前處理的方式，可以有效地降低在前處理時的人力、時間與有機溶劑的消耗，改良傳統前處理之缺點。

Polycyclic aromatic hydrocarbons (PAHs) and their derivatives are common trace pollutants produced during incomplete combustion of organic substances such as tobacco. After sampling cigarette smoke, PAH collators must undergo pretreatment processes such as extraction, cleanup and concentration before instrument analysis. This study combines molecular imprinted polymers (MIPs) and solid-phase extraction (SPE) to create a novel sample pretreatment technique.
Experimental results demonstrate that MIPs have good selectivity for Benz[a]Pyrene (BaP) from a mixture of 16 PAH solvents. The MIPs were applied to a sample taken from mainstream smoke from a cigarette. Based on functional monomers and cross-linkers, this study investigated three groups of MIPs. After the template was removed, various tests, including capacity, selectivity, recovery, scanning electron microscope (SEM) observations and real environmental sample tests, were conducted. Experimental results show that MIP-1 is the best MIP with a capacity of 20.78 ± 1.7 (μg/g), BaP selectivity and recovery exceeding 93%. In environmental sample tests, i.e., main stream smoke from S and M brand cigarettes, 80% of BAP was absorbed by MIP compared with that absorbed by filters. All experimental results suggest that MIPs effectively adsorbed BaP among 16 different PAHs, reduced background interference and increased signal resolution compared with traditional extraction techniques.
Additionally, using MIPs for sample pretreatment is less laborious than traditional sample pretreatments. It saves time and reduces organic solvent reduces consumption. Using molecular imprinted polymers solid extraction (MISPE) may separate target analytes from a complex sample more effectively than traditional Soxhlet extraction. Future toxicity tests should contribute to the understanding of toxic compounds emitted from specific pollution sources.

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