在CMRT相關的研究當中，透過動物實驗和組織切片的觀察，鈣基陶瓷對硬骨組織有顯著的修復效果。本研究的目的是透過原代培養的方式，從動物身上取出軟骨組織，經過取出軟骨組織、消化軟骨組織、原代培養和傳代培養這些步驟穩定地培養軟骨細胞，在未來把軟骨細胞種植在鈣基陶瓷材料，期待能夠研究出修復關節軟骨的新方式。

本研究培養軟骨細胞的方式為單層培養，具有經濟效益、操作較為簡單、又可以培養大量的細胞等優點；然而目前已經證明軟骨細胞在單層培養之下進行幾次傳代培養之後，會失去軟骨細胞原本的表型，若是轉移到三維培養環境中就不會使軟骨細胞失去表型。因此在未來，期待能夠以體外培養的方式，把鈣基陶瓷作為三維結構的生物支架，同時進行軟骨細胞的原代培養，嘗試讓軟骨細胞生長在陶瓷支架，並且把種植軟骨細胞的陶瓷支架植入動物，觀察是否會比純陶瓷支架的植入有更好的修復成果。

In one of the researches in CMRT (Cana Materials Research Team) Lab, calcium-based ceramic has shown excellent effect in animal experiments. Therefore, in this study, we tried to cultivate the chondrocytes by monolayer culture through acquiring cartilage tissue, digesting cartilage tissue, primary culture, and subculture. According to our research, the chondrocytes were harvested from the rib cartilage of Sprague Dawley rats and New Zealand rabbits by primary culture. In the future, we would like to make an attempt to seed chondrocytes on the bioceramic scaffold and then implant the joint defect to understand the effect of repairing cartilage.

The advantages of cultivating chondrocytes by monolayer culture is ecomonical and easier. Moreover, we can gain a large number of chondrocytes by comparing other cell culture methods. Nevertheless, it has been proved that chondrocytes would lose their phenotype by monolayer culture after subculture for several times. When these chondrocytes were transferred into the three-dimensional culture, chondrocytes recovered their phenotype. Because of the property of chondrocytes, we could try to seed chondrocytes on the three-dimensional bioceramic scaffold and then expect that the bioceramic scaffold cultivating with chondrocytes will implant the animal in the future.

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