本研究利用電場激發氣體形成大氣微電漿，透過電漿產生的活性氧物質(ROS)應用於生物醫療上，在一系列的研究中包含了促進細胞增生、抑制細菌生長、促進老鼠傷口癒合、促進具有糖尿病老鼠的傷口癒合、促進大型動物傷口癒合等等，最終目的是走向促進人體慢性傷口癒合，在了解慢性傷口之所以延遲癒合的其中一項原因與病原體的生長及其生物膜的存在相關，而在傳統治療方式是利用抗生素及清創來清潔傷口，當中未滿足的需求包含了抑制抗藥性病原體及其生物膜，因此希望透過電漿處理，達到抑制抗藥性病原體及其生物膜的方式，來降低傷口負載。
本研究電漿裝置是通入高純度He以及1% N2並以大約5 W的功率做能量源激發出電漿態，利用DIN的測量方式，獲得電漿各種產物的分析，在皮膚安全標準下，制定了5 mm的工作距離，並且在放射光譜的結果驗證了增加1% N2可以使電漿的ROS含量增加。
生物實驗為模擬慢性傷口並分成菌落及生物膜兩種情境，在菌落實驗結果觀察到電漿處理1分鐘後產生顯著差異，並對具有抗藥性白色念珠菌分別產生6 mm及存活率24%的抑菌圈，和抗藥性金黃色葡萄球菌產生9.5 mm及存活率大約6.8%的抑菌圈，透過生長速度來判斷病原體的適應能力，結果了解電漿處理菌相較於原菌的適應能力具有顯著的增加；在抗藥能力分析中，發現抑制圈內的細菌抗藥性能力在電漿循環處理第三次時產生顯著性的下降；在生物膜實驗結果表示2分鐘電漿處理後，念珠菌及金黃色葡萄球菌的平均菌數濃度分別減少2.38個Log及1.75個Log，達到臨床定義抑菌(bacteriostatic)的標準，並具有顯著性的差異，在電子顯微鏡觀察下兩種菌的表面形貌也因電漿處理而發生變化，透過實驗結果表明，低溫微電漿可以處理慢性傷口的細菌，可望未來可以發展成為處理慢性傷口的新選擇。

In this study, low-temperature micro-plasma with two working gases was used as the experimental equipment. First, DIN specifications were used to set the working parameters of the plasma system, so that the plasma products can be used in biomedicine. In biological experiments, drug-resistant Candida albicans and Staphylococcus aureus were analyzed the drug resistance and antibacterial effect after plasma treatment.
Measure plasma parameters through DIN regulations. The results of measuring plasma products show that the plasma working distance was set at 5mm and was in compliance with skin safety regulations. The results of biological experiments show that plasma treatment can significantly inhibit drug-resistant Candida albicans and Staphylococcus aureus after 1 min exposure time. In drug resistance test, it was found that the resistance of Staphylococcus aureus has significantly decreased after third plasma treatment cycle. In biofilm test, Candida albicans and Staphylococcus aureus biofilms can reduce average bacterial count 2 Log after 2 min plasma exposure time. It has a significant reduction and achieves bacteriostatic. After plasma treatment, it was observed that the surface morphology of the bacteria was changed in the SEM. The results show that plasma has antibacterial ability against drug-resistant Candida albicans and Staphylococcus aureus. It may be a new way for biological antibacterial in the future.

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