由於MEMS技術的成熟，使的微流體裝置能夠輕易的製作並且達到單一細胞的的研究等級(奈米)，本論文提出單一細胞分析方法，其比採取一群作分析提供了更精確及更深入的資訊對於生物細胞體的研究。阻抗量測是一種可以用來區分且分析不同細胞的特性的技術，更深入的地認識細胞個體差異，資訊傳遞以及藥物刺激之影
因此，本研究使用可操作與捕捉單一顆細胞的微流體晶片，針對四種不同的癌細胞進行阻抗量測，包含人類乳癌細胞 (MCF-7、 MDA-MB-231)、人類子宮頸癌細胞 (Hela) 和人類肺腺癌細胞 (A549)，藉由實驗結果顯示，可以看到四種細胞阻抗上的差異。同時建立單細胞等效電路模型去模擬細胞的阻抗，進而得到不同細胞之間在電特性上參數的差異。藉由實驗與模擬結果，探討隨著量測電壓的增加對細胞阻抗的影響，進而定出最佳的量測電壓去設計單細胞阻抗量測的微流體裝置

Electrical impedance spectroscopy (EIS) technique is used to analyze the character of electrochemical material for biomedical application. This paper presents a fast and low-cost manner to differentiate the four kinds of cells using impedance measurement in different voltage and frequency. Four kinds of cells (HeLa, MDA-MB-231, A549 and MCF-7) are analyzed and characterized on impedance by a trap-and-measure device. The impedance spectrums are obtained by the impedance analyzer at operating voltage of 0.2-1V and frequency from 20 to 101 kHz. According to the magnitude of impedance measurement, 0.4 V is the optimized operating voltage to distinguish the four kinds of cells most significantly. For the same cell line (MDA-MB-231 and MCF-7), the magnitude and phase are both distinguishable at 1.0V. In addition, an equivalent circuit model is established and fits with the experimental results. The circuit can be modeled electrically as cell impedance in parallel with capacitance of interfacial geometric per unit length of the electrode layout and in series with a pair of cell spreading resistance. The maximum decrease of the cytoplasm resistance occurs at different segments of operating voltage. For the A549 cell, the MDA-MB-231 cell, the MCF-7 cell and the HeLa cell, the maximum resistance variations are in 0.8-1.0V，0.8-1.0V，0.6-0.8V, and 0.4-0.6V, respectively. Because a strong electric filed may increase the exchange of ions between the cytoplasm and the culture medium.

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