具有適當表面性質之生醫材料可提供引導細胞生長的物理支撐。當細胞貼附在生物材料表面時，細胞主要感應三項理化因素：形貌、化學和機械的影響。在本研究中，選擇三種常用於生醫應用之生物材料，藉由創造不同的表面條件以評估與細胞表面交互作用關聯的表面性質。
在生物材料基材之研究中，以溶劑鑄造法製備具有平坦表面之緻密型聚乳酸，以氣泡成型法製備具有連通孔隙之多孔型聚乳酸。後者之孔隙間壁可提供細胞貼附之平坦表面且化學結構並無改變，但機械性質由於多孔結構而明顯降低。3T3纖維母細胞於緻密型聚乳酸表面貼附良好且產生纖維結構，此結果顯示細胞表面相互作用主要受到生物材料表面機械性質之影響，同時多孔結構亦提供更多細胞生長之路徑。
在改質表面的研究中，製備不同的自組裝單分子層吸附於金上。比較有序的十八烷基硫醇及十二碳烷硫醇分子由於它們的疏水性特徵而對於細胞貼附產生了不利影響，羧基硫醇提供親水性表面而能有效促進細胞貼附。十八烷基硫醇/金及十二碳烷硫醇/金有相同的尾端官能基並有相似的奈米力學性質且較羧基硫醇/金硬，但此奈米力學性質差異太過細微而不主導細胞貼附，因此化學因子成為細胞貼附於改質表面性質之主要因素。
在兩種不同表面的研究中，蒸鍍尺寸約150μm、厚度約20nm之微米金團簇於幾丁聚醣基材之上，實驗結果證實金團簇及其邊界區域有利於促進細胞貼附、攤附以及生長。金團簇與邊界區域之奈米硬度明顯增加而利於纖維母細胞聚集於金團簇及其邊界區域，此結果顯示細胞表面交互作用同時受到化學及機械性質之影響。
本研究顯示，具有不同表面特徵之生醫材料可提供細胞-表面交互作用相關之理化及力學因子(即形貌、化學、或機械性質)，透過表面性質控制可提供控制細胞貼附生長於特定區域之效能及發展性。

Biomedical materials with proper surface properties could provide physical support to guide cell growth. When cells attach to a biomaterial surface, they respond to three main physicochemical factors, namely topographical, chemical, and mechanical properties. In this study, three biomaterials that are generally used in biomedical applications are investigated. Surface properties associated with cell-surface interactions were assessed by creating distinct surface conditions.
In the study of biomaterial substrate, a dense poly L-lactic acid (PLLA) film fabricated using a solvent casting procedure exhibited a relatively smooth surface. A porous PLLA film fabricated using a gas forming procedure contained a variety of connected pores. For the latter, the connected wall among pores provided flat surfaces, enabling cell adhesion and the chemical structures of the porous PLLA film to remain unchanged. Nevertheless, the mechanical properties of the porous PLLA film were significantly lowered due to the porous structure. 3T3 fibroblast cells spread well and formed fibrous structures on the dense PLLA surface, which indicates that cell-surface interaction was influenced by the mechanical properties of the biomaterial substrate. Porous structures provided more pathways for cell growth.
In the study of modified surface, various self-assembled monolayers (SAMs) adsorbed on Au were prepared. The relatively ordered octadecanethiol (ODT) and dodecanethiol (DDT) molecules had an unfavorable effect on cell adhesion due to their hydrophobic characteristic. 11-mercaptoundecanoic acid (MUA) molecules provided a hydrophilic surface which significantly promoted cell adhesion. ODT/Au and DDT/Au had the same tail groups and their nano-mechanical properties were similar, both being stiffer than MUA/Au; however, the differences were too small to be a major factor in cell adhesion. The chemical properties were the main factor in cell adhesion for the modified surface.
In the study of two distinct surfaces, micro-scale Au clusters, ≒150 μm in diameter and ≒20 nm in thickness, were evaporated onto a chitosan substrate. Experimental results demonstrate that the Au clusters and their boundary area promoted cell adhesion, spreading, and growth. The nano-hardness on Au clusters and the boundary area significantly increased. Cultured fibroblast cells aggregated on the Au clusters and the boundary area, indicating that cell-surface interaction was influenced by both chemical and mechanical properties.
The results show that biomaterials with different surface characteristics have different physiochemical and mechanical properties (i.e., topographical, chemical, and mechanical properties) associated with cell-surface interaction and exactly have the efficiency and the development to influence cell adhesion and proliferation upon the specific region via proper surface property control.

[1] S. A. Riboldi, M. Sampaolesi, P. Neuenschwander, G. Cossu, and S. Mantero, "Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering," Biomaterials, Vol. 26, pp. 4606-4615, 2005.
[2] I. K. Park, J. Yang, H. J. Jeong, H. S. Bom, I. Harada, T. Akaike, S. I. Kim, and C. S. Cho, "Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment," Biomaterials, Vol. 24, pp. 2331-2337, 2003.
[3] F. Yang, R. Murugan, S. Ramakrishna, X. Wang, Y. X. Ma, and S. Wang, "Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering," Biomaterials, Vol. 25, pp. 1891-1900, 2004.
[4] M. Borden, S. F. El-Amin, M. Attawia, and C. T. Laurencin, "Structural and human cellular assessment of a novel microsphere-based tissue engineered scaffold for bone repair," Biomaterials, Vol. 24, pp. 597-609, 2003.
[5] Y. C. Toh, S. T. Ho, Y. Zhou, D. W. Hutmacher, and H. Yu, "Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system," Biomaterials, Vol. 26, pp. 4149-4160, 2005.
[6] I. Adekogbe and A. Ghanem, "Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering," Biomaterials, Vol. 26, pp. 7241-7250, 2005.
[7] K. S. Chen, Y. A. Ku, C. H. Lee, H. R. Lin, F. H. Lin, and T. M. Chen, "Immobilization of chitosan gel with cross-linking reagent on PNIPAAm gel/PP nonwoven composites surface," Materials Science and Engineering: C, Vol. 25, pp. 472-478, 2005.
[8] L. Ma, C. Gao, Z. Mao, J. Zhou, J. Shen, X. Hu, and C. Han, "Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering," Biomaterials, Vol. 24, pp. 4833-4841, 2003.
[9] C. A. Stone, H. Wright, T. Clarke, R. Powell, and V. S. Devaraj, "Healing at skin graft donor sites dressed with chitosan," British Journal of Plastic Surgery, Vol. 53, pp. 601-606, 2000.
[10] G. R. Evans, "Challenges to nerve regeneration," Seminars in Surgical Oncology, Vol. 19, pp. 312-318, 2000.
[11] H. Shin, S. Jo, and A. G. Mikos, "Biomimetic materials for tissue engineering," Biomaterials, Vol. 24, pp. 4353-4364, 2003.
[12] P. Sarazin, X. Roy, and B. D. Favis, "Controlled preparation and properties of porous poly(L-lactide) obtained from a co-continuous blend of two biodegradable polymers," Biomaterials, Vol. 25, pp. 5965-5978, 2004.
[13] B. A. Harley, J. H. Leung, E. C. C. M. Silva, and L. J. Gibson, "Mechanical characterization of collagen-glycosaminoglycan scaffolds," Acta Biomaterialia, Vol. 3, pp. 463-474, 2007.
[14] H. Tan, J. Wu, L. Lao, and C. Gao, "Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering," Acta Biomaterialia, Vol. 5, pp. 328-337, 2009.
[15] D. D. Allison, N. Vasco, K. R. Braun, T. N. Wight, and K. Jane Grande-Allen, "The effect of endogenous overexpression of hyaluronan synthases on material, morphological, and biochemical properties of uncrosslinked collagen biomaterials," Biomaterials, Vol. 28, pp. 5509-5517, 2007.
[16] C. L. Huang, J. D. Liao, C. F. Yang, C. W. Chang, M. S. Ju, and C. C. K. Lin, "Cell adhesion over two distinct surfaces varied with chemical and mechanical properties," Thin Solid Films, Vol. 517, pp. 5386-5389, 2009.
[17] Y. L. Cui, A. D. Qi, W. G. Liu, X. H. Wang, H. Wang, D. M. Ma, and K. D. Yao, "Biomimetic surface modification of poly(L-lactic acid) with chitosan and its effects on articular chondrocytes in vitro," Biomaterials, Vol. 24, pp. 3859-3868, 2003.
[18] A. K. Pannier, J. A. Wieland, and L. D. Shea, "Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes," Acta Biomaterialia, Vol. 4, pp. 26-39, 2008.
[19] Y. Arima and H. Iwata, "Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers," Biomaterials, Vol. 28, pp. 3074-3082, 2007.
[20] M. H. Lee, P. Ducheyne, L. Lynch, D. Boettiger, and R. J. Composto, "Effect of biomaterial surface properties on fibronectin-α5β1 integrin interaction and cellular attachment," Biomaterials, Vol. 27, pp. 1907-1916, 2006.
[21] S. Yokota, T. Kitaoka, and H. Wariishi, "Biofunctionality of self-assembled nanolayers composed of cellulosic polymers," Carbohydrate Polymers, Vol. 74, pp. 666-672, 2008.
[22] M. M. Stevens and J. H. George, "Exploring and Engineering the Cell Surface Interface " Science, Vol. 310, pp. 1135-1138, 2005.
[23] A. Kikuchi and T. Okano, "Nanostructured designs of biomedical materials: applications of cell sheet engineering to functional regenerative tissues and organs," Journal of Controlled Release, Vol. 101, pp. 69-84, 2005.
[24] J. Y. Wong, J. B. Leach, and X. Q. Brown, "Balance of chemistry, topography, and mechanics at the cell-biomaterial interface: Issues and challenges for assessing the role of substrate mechanics on cell response," Surface Science, Vol. 570, pp. 119-133, 2004.
[25] M. Dalkiz, H. S. Gokce, A. Aydin, and B. Beydemir, "Gold weight implantation for rehabilitation of the paralysed eyelid," International Journal of Oral and Maxillofacial Surgery, Vol. 36, pp. 522-526, 2007.
[26] F. A. Petrigliano, D. R. McAllister, and B. M. Wu, "Tissue Engineering for Anterior Cruciate Ligament Reconstruction: A Review of Current Strategies," Arthroscopy: The Journal of Arthroscopic & Related Surgery, Vol. 22, pp. 441-451, 2006.
[27] I. V. Yannas, "Synthesis of tissues and organs," Chembiochem, Vol. 5, pp. 26-39, 2004.
[28] H. J. Chung and T. G. Park, "Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering," Advanced Drug Delivery Reviews, Vol. 59, pp. 249-262, 2007.
[29] B. L. Seal, T. C. Otero, and A. Panitch, "Polymeric biomaterials for tissue and organ regeneration," Materials Science and Engineering: R: Reports, Vol. 34, pp. 147-230, 2001.
[30] X. Zong, H. Bien, C. Y. Chung, L. Yin, D. Fang, B. S. Hsiao, B. Chu, and E. Entcheva, "Electrospun fine-textured scaffolds for heart tissue constructs," Biomaterials, Vol. 26, pp. 5330-5338, 2005.
[31] X. M. Mo, C. Y. Xu, M. Kotaki, and S. Ramakrishna, "Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation," Biomaterials, Vol. 25, pp. 1883-1890, 2004.
[32] X. Liu, Y. Won, and P. X. Ma, "Porogen-induced surface modification of nano-fibrous poly(L-lactic acid) scaffolds for tissue engineering," Biomaterials, Vol. 27, pp. 3980-3987, 2006.
[33] V. J. Chen and P. X. Ma, "Nano-fibrous poly(L-lactic acid) scaffolds with interconnected spherical macropores," Biomaterials, Vol. 25, pp. 2065-2073, 2004.
[34] G. R. Owen, J. Jackson, B. Chehroudi, H. Burt, and D. M. Brunette, "A PLGA membrane controlling cell behavior for promoting tissue regeneration," Biomaterials, Vol. 26, pp. 7447-7456, 2005.
[35] S. Liao, W. Wang, M. Uo, S. Ohkawa, T. Akasaka, K. Tamura, F. Cui, and F. Watari, "A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration," Biomaterials, Vol. 26, pp. 7564-7571, 2005.
[36] C. Berkland, D. W. Pack, and K. K. Kim, "Controlling surface nano-structure using flow-limited field-injection electrostatic spraying (FFESS) of poly (D,L-lactide-co-glycolide)," Biomaterials, Vol. 25, pp. 5649-5658, 2004.
[37] M. Hakkarainen, A. C. Albertsson, and S. Karlsson, "Weight losses and molecular weight changes correlated with the evolution of hydroxyacids in simulated in vivo degradation of homo- and copolymers of PLA and PGA," Polymer Degradation and Stability, Vol. 52, pp. 283-291, 1996.
[38] Z. Ma, C. Gao, Y. Gong, and J. Shen, "Chondrocyte behaviors on poly--lactic acid (PLLA) membranes containing hydroxyl, amide or carboxyl groups," Biomaterials, Vol. 24, pp. 3725-3730, 2003.
[39] Y. Kang, Y. Yao, G. Yin, Z. Huang, X. Liao, X. Xu, and G. Zhao, "A study on the in vitro degradation properties of poly (L-lactic acid) / β-tricalcuim phosphate (PLLA/β-TCP) scaffold under dynamic loading," Medical Engineering & Physics, Vol. 31, pp. 589-594, 2009.
[40] B. M. Min, S. W. Lee, J. N. Lim, Y. You, T. S. Lee, P. H. Kang, and W. H. Park, "Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers," Polymer, Vol. 45, pp. 7137-7142, 2004.
[41] Y. W. Cho, Y. N. Cho, S. H. Chung, G. Yoo, and S. W. Ko, "Water-soluble chitin as a wound healing accelerator," Biomaterials, Vol. 20, pp. 2139-2145, 1999.
[42] K. Tomihata and Y. Ikada, "In vitro and in vivo degradation of films of chitin and its deacetylated derivatives," Biomaterials, Vol. 18, pp. 567-575, 1997.
[43] F. L. Mi, S. S. Shyu, Y. B. Wu, S. T. Lee, J. Y. Shyong, and R. N. Huang, "Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing," Biomaterials, Vol. 22, pp. 165-173, 2001.
[44] S. V. Madihally and H. W. T. Matthew, "Porous chitosan scaffolds for tissue engineering," Biomaterials, Vol. 20, pp. 1133-1142, 1999.
[45] C. J. Knill, J. F. Kennedy, J. Mistry, M. Miraftab, G. Smart, M. R. Groocock, and H. J. Williams, "Alginate fibres modified with unhydrolysed and hydrolysed chitosans for wound dressings," Carbohydrate Polymers, Vol. 55, pp. 65-76, 2004.
[46] H. Hong, J. Wei, and C. Liu, "Development of asymmetric gradational-changed porous chitosan membrane for guided periodontal tissue regeneration," Composites Part B: Engineering, Vol. 38, pp. 311-316, 2007.
[47] Y. Yuan, P. Zhang, Y. Yang, X. Wang, and X. Gu, "The interaction of Schwann cells with chitosan membranes and fibers in vitro," Biomaterials, Vol. 25, pp. 4273-4278, 2004.
[48] Z. Ding, J. Chen, S. Gao, J. Chang, J. Zhang, and E. T. Kang, "Immobilization of chitosan onto poly--lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells," Biomaterials, Vol. 25, pp. 1059-1067, 2004.
[49] T. Mori, M. Okumura, M. Matsuura, K. Ueno, S. Tokura, Y. Okamoto, S. Minami, and T. Fujinaga, "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro," Biomaterials, Vol. 18, pp. 947-951, 1997.
[50] J. N. Barbosa, M. A. Barbosa, and A. P. Aguas, "Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold," Biomaterials, Vol. 25, pp. 2557-2563, 2004.
[51] B. O. Palsson and S. Bhatia, Tissue Engineering. London: Pearson Education, Inc., 2004.
[52] J. S. Mao, Y. L. Cui, X. H. Wang, Y. Sun, Y. J. Yin, H. M. Zhao, and K. De Yao, "A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis," Biomaterials, Vol. 25, pp. 3973-3981, 2004.
[53] Y. Wan, Y. Wang, Z. Liu, X. Qu, B. Han, J. Bei, and S. Wang, "Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(L-lactide)," Biomaterials, Vol. 26, pp. 4453-4459, 2005.
[54] C. C. Berry, G. Campbell, A. Spadiccino, M. Robertson, and A. S. G. A. S. G. Curtis, "The influence of microscale topography on fibroblast attachment and motility," Biomaterials, Vol. 25, pp. 5781-5788, 2004.
[55] C. C. Berry, M. J. Dalby, D. McCloy, and S. Affrossman, "The fibroblast response to tubes exhibiting internal nanotopography," Biomaterials, Vol. 26, pp. 4985-4992, 2005.
[56] J. L. Charest, L. E. Bryant, A. J. Garcia, and W. P. King, "Hot embossing for micropatterned cell substrates," Biomaterials, Vol. 25, pp. 4767-4775, 2004.
[57] J. S. Goldner, J. M. Bruder, G. Li, D. Gazzola, and D. Hoffman-Kim, "Neurite bridging across micropatterned grooves," Biomaterials, Vol. 27, pp. 460-472, 2006.
[58] J. B. Recknor, D. S. Sakaguchi, and S. K. Mallapragada, "Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates," Biomaterials, Vol. 27, pp. 4098-4108, 2006.
[59] J. L. Charest, M. T. Eliason, A. J. Garcia, and W. P. King, "Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates," Biomaterials, Vol. 27, pp. 2487-2494, 2006.
[60] C. H. Choi, S. H. Hagvall, B. M. Wu, J. C. Y. Dunn, R. E. Beygui, and C. J. Kim, "Cell interaction with three-dimensional sharp-tip nanotopography," Biomaterials, Vol. 28, pp. 1672-1679, 2007.
[61] N. Gadegaard, E. Martines, M. O. Riehle, K. Seunarine, and C. D. W. Wilkinson, "Applications of nano-patterning to tissue engineering," Microelectronic Engineering, Vol. 83, pp. 1577-1581, 2006.
[62] C. D. W. Wilkinson, M. Riehle, M. Wood, J. Gallagher, and A. S. G. Curtis, "The use of materials patterned on a nano- and micro-metric scale in cellular engineering," Materials Science and Engineering: C, Vol. 19, pp. 263-269, 2002.
[63] Y. Chen, A. F. T. Mak, M. Wang, J. Li, and M. S. Wong, "PLLA scaffolds with biomimetic apatite coating and biomimetic apatite/collagen composite coating to enhance osteoblast-like cells attachment and activity," Surface and Coatings Technology, Vol. 201, pp. 575-580, 2006.
[64] F. Schreiber, "Structure and growth of self-assembling monolayers," Progress in Surface Science, Vol. 65, pp. 151-257, 2000 2000.
[65] R. K. Smith, P. A. Lewis, and P. S. Weiss, "Patterning self-assembled monolayers," Progress in Surface Science, Vol. 75, pp. 1-68, 2004.
[66] E. V. Romanova, S. P. Oxley, S. S. Rubakhin, P. W. Bohn, and J. V. Sweedler, "Self-assembled monolayers of alkanethiols on gold modulate electrophysiological parameters and cellular morphology of cultured neurons," Biomaterials, Vol. 27, pp. 1665-1669, 2006.
[67] D. E. Discher, P. Janmey, and Y. l. Wang, "Tissue Cells Feel and Respond to the Stiffness of Their Substrate " Science, Vol. 310, pp. 1139-1143, 2005.
[68] X. Q. Brown, K. Ookawa, and J. Y. Wong, "Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell response," Biomaterials, Vol. 26, pp. 3123-3129, 2005.
[69] K. Ghosh, Z. Pan, E. Guan, S. Ge, Y. Liu, T. Nakamura, X.-D. Ren, M. Rafailovich, and R. A. F. Clark, "Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties," Biomaterials, Vol. 28, pp. 671-679, 2007.
[70] D. S. Gray, J. Tien, and C. S. Chen, "Repositioning of cells by mechanotaxis on surfaces with micropatterned Young's modulus," Journal of Biomedical Materials Research Part A, Vol. 66A, pp. 605-614, 2003.
[71] C. M. Lo, H. B. Wang, M. Dembo, and Y. l. Wang, "Cell Movement Is Guided by the Rigidity of the Substrate," Biophysical Journal, Vol. 79, pp. 144-152, 2000.
[72] S. R. Peyton and A. J. Putnam, "Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion," Journal of Cellular Physiology, Vol. 204, pp. 198-209, 2005.
[73] S. R. Peyton, C. B. Raub, V. P. Keschrumrus, and A. J. Putnam, "The use of poly(ethylene glycol) hydrogels to investigate the impact of ECM chemistry and mechanics on smooth muscle cells," Biomaterials, Vol. 27, pp. 4881-4893, 2006.
[74] W. Timp, P. Matsudaira, and Dr. John J. Correia and Dr. H. William Detrich, III, "Electron Microscopy of Hydrated Samples," in Methods in Cell Biology. vol. 89: Academic Press, 2008, pp. 391-407.
[75] B. D. Cullity and S. R. Stock, Elements of X-ray diffraction. Upper Saddle River, NJ Prentice Hall, 2001.
[76] P. Colombi, P. Zanola, E. Bontempi, and L. E. Depero, "Modeling of glancing incidence X-ray for depth profiling of thin layers," Spectrochimica Acta Part B: Atomic Spectroscopy, Vol. 62, pp. 554-557, 2007.
[77] W. Tang, L. Deng, K. Xu, and J. Lu, "X-ray diffraction measurement of residual stress and crystal orientation in Au/NiCr/Ta films prepared by plating," Surface and Coatings Technology, Vol. 201, pp. 5944-5947, 2007.
[78] D. K. Sarkar, S. Dhara, K. G. M. Nair, and S. Chowdhury, "Studies of phase formation and chemical states of the ion beam mixed Ag/Si(111) system," Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 168, pp. 215-220, 2000.
[79] D. K. Sarkar, S. Dhara, A. Gupta, K. G. M. Nair, and S. Chaudhury, "Structural instability of the ion beam-mixed Au/Si(111) systems at elevated temperatures," Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 168, pp. 21-28, 2000.
[80] D. K. Sarkar, S. Bera, S. Dhara, S. V. Narasimhan, S. Chowdhury, and K. G. M. Nair, "GIXRD and XPS study of the ion beam mixed Au/Si(111) system," Solid State Communications, Vol. 105, pp. 351-356, 1998.
[81] A. Sarapuu, M. Nurmik, H. Mandar, A. Rosental, T. Laaksonen, K. Kontturi, D. J. Schiffrin, and K. Tammeveski, "Electrochemical reduction of oxygen on nanostructured gold electrodes," Journal of Electroanalytical Chemistry, Vol. 612, pp. 78-86, 2008.
[82] G. M. Odegard, T. S. Gates, and H. M. Herring, "Characterization of viscoelastic properties of polymeric materials through nanoindentation " Experimental Mechanics, Vol. 45, pp. 1741-2765, 2005.
[83] G. M. Pharr, W. C. Oliver, and F. R. Brotzen, "On the Generality of the Relationship Among Contact Stiffness, Contact Area, and Elastic Modulus During Indentation," Journal of Materials Research, Vol. 7, pp. 613-617, 1992.
[84] A. Bourmaud and C. Baley, "Rigidity analysis of polypropylene/vegetal fibre composites after recycling," Polymer Degradation and Stability, Vol. 94, pp. 297-305, 2009.
[85] M. A. G. Maneiro and J. Rodriguez, "Pile-up effect on nanoindentation tests with spherical-conical tips," Scripta Materialia, Vol. 52, pp. 593-598, 2005.
[86] J. Thurn and R. F. Cook, "Simplified area function for sharp indenter tips in depth-sensing indentation," Journal of Materials Research, Vol. 17, pp. 1143-1146, 2002.
[87] B. Sandip, W. B. Michel, D. W. Adrian, and T. D. Moustakas, "Spherical nanoindentation and deformation mechanisms in freestanding GaN films," Journal of Applied Physics, Vol. 101, p. 083522, 2007.
[88] Y. Lu and S. C. Chen, "Micro and nano-fabrication of biodegradable polymers for drug delivery," Advanced Drug Delivery Reviews, Vol. 56, pp. 1621-1633, 2004.
[89] G. R. Evans, "Challenges to nerve regeneration," Seminars in Surgical Oncology, Vol. 19, pp. 312-8, 2000.
[90] Y. L. Cui, A. D. Qi, W. G. Liu, X. H. Wang, H. Wang, D. M. Ma, and K. D. Yao, "Biomimetic surface modification of poly(-lactic acid) with chitosan and its effects on articular chondrocytes in vitro," Biomaterials, Vol. 24, pp. 3859-3868, 2003.
[91] V. J. Chen and P. X. Ma, "Nano-fibrous poly(-lactic acid) scaffolds with interconnected spherical macropores," Biomaterials, Vol. 25, pp. 2065-2073, 2004.
[92] P. Sarazin, X. Roy, and B. D. Favis, "Controlled preparation and properties of porous poly(-lactide) obtained from a co-continuous blend of two biodegradable polymers," Biomaterials, Vol. 25, pp. 5965-5978, 2004.
[93] J. J. Yoon and T. G. Park, "Degradation behaviors of biodegradable macroporous scaffolds prepared by gas foaming of effervescent salts," Journal of Biomedical Materials Research, Vol. 55, pp. 401-408, 2001.
[94] K. B. McClary and D. W. Grainger, "RhoA-induced changes in fibroblasts cultured on organic monolayers," Biomaterials, Vol. 20, pp. 2435-2446, 1999.
[95] C. C. Weng, J. D. Liao, Y. T. Wu, M. C. Wang, R. Klauser, and M. Zharnikov, "Modification of Monomolecular Self-Assembled Films by Nitrogen-Oxygen Plasma," The Journal of Physical Chemistry B, Vol. 110, pp. 12523-12529, 2006.
[96] P. Kwok, M. Schuster, K. Boch, P. Jacob, O. Gleich, and J. Strutz, "Safety of gold in stapes surgery," Biomaterials, Vol. 26, pp. 7132-7135, 2005.
[97] S. Ghosh, J. i. C. Viana, R. L. Reis, and J. F. Mano, "Development of porous lamellar poly(l-lactic acid) scaffolds by conventional injection molding process," Acta Biomaterialia, Vol. 4, pp. 887-896, 2008.
[98] D. E. Discher, P. Janmey, and Y.-l. Wang, "Tissue Cells Feel and Respond to the Stiffness of Their Substrate " Science, Vol. 310, pp. 1139-1143, 2005.
[99] C.-M. Lo, H.-B. Wang, M. Dembo, and Y.-l. Wang, "Cell Movement Is Guided by the Rigidity of the Substrate," Biophysical Journal, Vol. 79, pp. 144-152, 2000.
[100] M. Nakajima, T. Ishimuro, K. Kato, I.-K. Ko, I. Hirata, Y. Arima, and H. Iwata, "Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation," Biomaterials, Vol. 28, pp. 1048-1060, 2007.
[101] N. Faucheux, R. Schweiss, K. Lutzow, C. Werner, and T. Groth, "Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies," Biomaterials, Vol. 25, pp. 2721-2730, 2004.
[102] F. Brandl, F. Sommer, and A. Goepferich, "Rational design of hydrogels for tissue engineering: Impact of physical factors on cell behavior," Biomaterials, Vol. 28, pp. 134-146, 2007.
[103] C. C. Weng, J. D. Liao, Y. T. Wu, M. C. Wang, R. Klauser, M. Grunze, and M. Zharnikov, "Modification of Aliphatic Self-Assembled Monolayers by Free-Radical-Dominant Plasma: The Role of the Plasma Composition," Langmuir, Vol. 20, pp. 10093-10099, 2004.
[104] C. L. Huang, C. W. Chang, J. D. Liao, Y. T. Wu, M. S. Ju, and C. C. K. Lin, "Cells anchored upon a thin organic film with different nano-mechanical properties," Applied Surface Science, Vol. 255, pp. 301-303, 2008.
[105] C. H. Chang, J. D. Liao, J. J. J. Chen, M. S. Ju, and C. C. Lin, "Alkanethiolate self-assembly monolayers upon Au-deposited nerve microelectrode: cell adhesion examined by total impedance and cell detachment force," Nanotechnology, Vol. 17, pp. 2449-2457, 2006.
[106] M. F. Toney and T. C. Huang, "X-ray depth profiling of iron oxide thin films," Journal of Materials Research, Vol. 3, pp. 351-356, 1988.