針對低體積量樣本內生物樣本的分離與檢測，在研發上一直都是熱門話題。以微流體晶片為基礎，對於含有單種或複數種的生物粒子開發出間具有分離、濃縮、線上可量化功能者，因更符合實用化的需求之故，近兩、三年來，更屢有佳作被揭露。傳統欲進行人體樣本的物質檢測需要藉由生化的方法(免疫分析、螢光呈色)，且需將雜質或干擾物去除，以利進行檢測物質的純化，其花費的時間與金錢非常冗長且昂貴，故如何將上述的步驟簡化與加速是現今檢測平台要專注的課題之一。本研究提出，藉由交流電的非均勻電場產生的波形微結構結合交流電滲流的生物粒子操控與即時檢測的微流體晶片，以達到快速檢測、靈敏度高且不需生物標定的生物感測晶片。在波形結構上，藉由使用交流電產生的非均勻電場使在電極上的紫外線固化膠產生波浪狀的立體結構，波浪結構會隨著電場的強弱差別形成波峰或波谷。之後再搭配上介電泳以及交流電滲流的力量，產生出不同於平常的介電泳與交流電滲流現象。本研究運用在波形結構波峰上的介電泳和交流電滲流力量平衡使粒子可以產生混合的效果，這樣的現象可以應用在有修飾的抗原抗體粒子之間的結合效果加強，不同於以往的結合是分子之間的結合係數探討。 研究中使用Staphylococcus aureus 與antibody IgG修飾的粒子，在我們的晶片中進行混合後，利用兩者之間鍵結力量產生凝集的反應，在頻率的調控下可以觀察到有凝集與無凝集的Staphylococcus aureus 的區別。本研究提供一個粒子混合的平台藉由不同粒子表面的修飾便可以進行特定細菌的捕捉與偵測。

In recent years, a lot of research group were conducting work on isolation and detection of biological sample. There were many developing method with variety of different technologies for the particular biological particles in the separation stage. In this study, we proposed the combination of the micro-ripple structure generated by non-uniform electric field and particle manipulation by electrodynamics to achieve rapid detection, high sensitivity and without biological calibration biochips. The micro-ripple structure was made of ultraviolet-curable adhesive that used the UV oven to cure. The structure combined with the DEP and ACEO drag force which have different from the usual dielectrokinetics. In this study, the balance of DEP and ACEO drag force at the crest of micro-ripple structures, so that the particles can produce mixing results. The phoneme can be applied by the combine effects strengthen of the modified antigen-antibody on particles and investigated the combination coefficients. Staphylococcus aureus and Anti-protein A IgG used in a mixing chip study, the agglutination reactions generated based on the bonding strength. Under the frequency control, we can observe difference with and without agglutination of Staphylococcus aureus. This study provides a new platform by mixing particles of different particle surface modification can make a specific capture and detection of bacteria.

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