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| 研究生： |
許峻嘉 Xu, Jun-Jia |
|---|---|
| 論文名稱： |
綠色螢光蛋白發光團之間位吡啶類似物的合成及光/熱異構化性質及模擬GFP於酸性條件下的命運 meta-Pyridinium Chromophores of GFP Analogue : Synthesis, Photo/Thermoisomerization and Simulation the Fate of the GFP under Acidic Condition |
| 指導教授： |
宋光生
Sung, Kuang-Sen |
| 學位類別： |
碩士 Master |
| 系所名稱： |
理學院 - 化學系 Department of Chemistry |
| 論文出版年： | 2017 |
| 畢業學年度： | 105 |
| 語文別： | 中文 |
| 論文頁數： | 110 |
| 中文關鍵詞： | 綠色螢光蛋白 、甲基化 、酸性條件 、水解 |
| 外文關鍵詞： | GFP, methylation, acidic condition, hydrolyze |
| 相關次數： | 點閱：159 下載：11 |
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綠色螢光蛋白的研究大多取自生物體內，所耗的成本高且產率極低，過去的文獻顯示，綠色螢光蛋白於酸性條件下性質會發生改變，但其中發生的反應機制卻尚未明朗，本實驗利用合成綠色螢光蛋白類似物，進一步甲基化後去模擬綠色螢光蛋白(p-HBDI)於酸性條件下的結果。藉由類似物(m3-ph、m3-bn)及甲基化後類似物(m4-ph、m4-bn)去比較前後電子密度的差異，及類似物的光物理性質的測量，另外探討溶劑對光/熱異構化所造成的影響，並利用sigma-plot軟體計算速率常數，最後經過多項數據和x-ray的證實，綠色螢光蛋白類似物於酸性條件中，反應將發生在C9位置上。
In order to realize the chromophore of green fluorescent protein (p-HBDI) reacting under acidic condition, we used GFP chromophore analogues (m4-ph and m4-bn) to imitate p-HBDI. The m4-ph and m4-bn were synthesized by methylation method. Herein, we studied the effect of photo/thermoisomerization in different solvent and observed the integration value of 1H-NMR to calculate the rate of photo/thermoisomerization with sigma-plot. After methylation method, we found that electron density on carbons of GFP chromophoric analogues (m4-ph and m4-bn) changed due to the positively charged nitrogen. Because the C9 became more electron deficient, it will be attacked by nucleophile. The futher use of x-ray diffraction proved that m4-ph and m4-bn would hydrolyze in the end. These data show that the destiny of GFP chromophoric analogues (m4-ph and m4-bn) will reacted on the C9 under acidic condition finally.
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校內：2020-01-01公開校外：不公開