十八世紀以來，工業技術與科技的進步使化石燃料被快速消耗，伴隨著對環境的嚴重污染，人類對能源的需求也隨之增長，開發新興且能永續發展的綠色能源成為國際間重要的課題之一。其中，生質酒精被多數科學家認為是現階段具前瞻性的再生能源之一，然而，現有科技僅能利用纖維性生質體(Biomass)中約百分之七十五到八十的電子生產生質酒精，換句話說，約百分之二十到二十五的能量會作為殘渣廢棄。本研究的目的便是期望將纖維生質酒精的發酵殘渣進一步回收，並同時解決生質能源工業化時之環保問題。
本研究建置一兩階段式生物程序用以處理纖維生質酒精發酵殘渣，串聯兩個CSTR 厭氧發酵反應槽，分別生產氫氣與甲烷。經由特性分析，纖維酒精發酵殘渣的COD約為23 g/L，其中百分之二十五為碳水化合物，乳酸及乙酸則分別佔有百分之十六及十四。氫發酵槽在操作四個試程之後，發現體積負荷(VLR)為46 kg COD/m3/day時有最好的產氫速率與轉化率，分別為24.95 mL H2/g VSS/hr和20.3 mL H2/g COD。在厭氧產氫的批次實驗中，基質負荷(S0/X0)與乙酸化(acetogenesis)被認為是影響產氫表現的重要因子。而在甲烷發酵槽方面，當體積負荷為4.6 kg COD/m3/day的時候，能有最高的產氣速率1.16 L CH4/L/day。纖維生質酒精殘渣中，約有百分之七十五的COD能在兩階段發酵程序中被去除，其中百分之一點三轉化為氫氣，百分之六十六則轉為甲烷，顯示此程序能將纖維生質酒精殘渣做生質能源的回收再利用，而且，對操作條件的調整將能進一步提升氫氣與甲烷的生成與回收效率。

Bioethanol, which is produced from biomass, has become one of the most promising renewable “green energy” since scientists realized the fast consumption of fossil fuels and severe pollutions after using them. Current technologies can only utilize 75-80% of the energy during cellulosic bioethanol fermentation, which implies that 20-25% might be wasted as residues. Thus, this study has focused on the energy recovery from cellulosic bioethanol residues.
A two-stage bioprocess treating cellulosic bioethanol residues for recovering bioenergy, in the forms of hydrogen and methane, was conducted in the study. Nearly 25% of COD in cellulosic bioethanol residues was carbohydrate, and acetate and lactate accounted for around 14% and 16%, respectively. The maximum specific H2 production rate and yield were investigated to be 24.95 mL H2/g VSS/hr and 20.3 mL H2/g COD when volumetric loading rate (VLR) was 46 kg COD/m3/day. By carrying out batch experiment, acetogenesis and S0/X0, or F/M ratio in CSTR, were found to be important factors on the efficiency of biohydrogen production. The best performance of the methane bioreactor in the view of methane production was obtained under VLR of 4.6 kg COD/m3/day, in which 1.16 L CH4/L/day and 68.6 mL CH4/g VSS/hr were achieved. Approximately 75% of the COD in the bioethanol-fermentation residues was removed, with about 1.3% and 66% of it were recovered in the forms of hydrogen and methane. Results indicate that cellulosic bioethanol residues are also suitable for hydrogen and methane fermentation, but an optimized operational condition is still needed.

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