幾丁聚醣(Chitosan)在自然界中的含量僅次於纖維素，結構上近似於氨基葡聚醣(glycoaminoglycan，GAGs)，氨基葡聚醣是生物體中細胞外基質的主要成分之一。與氨基葡聚醣相較下，幾丁聚醣成本較低廉，因此逐漸被應用在組織工程的領域中。由於幾丁聚醣分子上帶正電的氨基(amino group)被認為是幾丁聚醣在細胞培養上獨特生化特性的主要因素，因此本研究在幾丁聚醣第二個碳上的氨基進行保護與去保護策略，並進一步嘗試將幾丁聚醣第六個碳上的羥基，導入磷酸根官能基，以模仿細胞膜磷脂質的雙電性特性。

由實驗XPS以及ATR-FTIR的結果，顯示磷酸根官能基已經成功接枝於幾丁聚醣上，且表面氨基之保護與去保護策略也有成效，接觸角分析也得知磷酸化後表面會變得較為親水，SEM觀察其表面形態亦非常的平整。

磷酸化後的幾丁聚醣和血液接觸後，意外的發現其對於血小板吸附有促進的效果，且也能縮短血漿再鈣化的時間，將來於凝血方面的應用指日可待。

Chitosan is the second most abundant nature polysaccharide having a similar structure as glycosaminoglycans (GAGs) which are the main components of extra cellular matrix (ECM). Due to its relatively lower cost as compared to GAGs, many researches had tried to look for the application of chitosan in tissue engineering. Because the amino group at C2 of chitosan is thought as the major positive functional group with special biological characteristics in cell culture, it should be protected to maintain its original properties.

In this study, we follow the protection-deprotection strategy to keep the amount of the amino groups and then grafted the phosphorous groups onto the chitosan film. After this procedure, hopefully we could imitate the zwetterionic characteristics of phospholipids of the biomembrane.

From the results of ESCA and ATR-FTIR, we can tell the phosphorous group had been successfully grafted onto the chitosan film and the protection-deprotection strategy also worked. Static contact angle showed that the phosphorylated chitosan became more hydrophilic and the surface morphology was still very smooth and homogeneous.

What’s more, in the blood compatibility aspect, phosphorylated chitosan films revealed more platelets adhered onto it and less plasma recalcification time. In the future, the phosphorylated chitosan film may be used as a biomaterial to improve blood coagulation.

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