分子模版聚合物 (Molecularly imprinted polymer，MIP) 是一種具有選擇辨識能力的合成巨分子結構，其能力之來源，乃是因進行分子拓印後，所形成的許多具有特定立體結構與化學作用力的孔洞。MIP 可應用於許多領域：如製作感測器、層析分離與作為觸媒使用等。
在本研究中所使用的目標分子是牛血清白蛋白 (Bovine serum albumin，BSA)，它與人類血清白蛋白 (Human serum albumin，HSA) 在生理或物理特性上都有極高的相似度。HSA 是血液中蛋白質物種含量最高者，主要是作為體內養分或代謝廢物的攜帶者。當人類體液 (血液或尿液) 中 HSA 含量發生變化時，即表示人體可能患有某些疾病。因此對 HSA 有選擇辨識能力的 MIP，有應用於臨床診斷上的可能性。
在本研究中所合成的 MIP 有兩種，第一種是以官能單體 (3-二甲胺丙基) 甲基丙烯醯胺 (3-Dimethylaminopropyl methacrylamide，DMAPMA) 搭配具有線形分子結構交聯劑：四甘醇二甲基丙烯酸酯 (Tetraethyleneglycol dimethacrylate，TEGDMA) 所製得之 MIP 。在此一合成 MIP 實驗中發現：當以水分量為 6.04％ 的 DMAPMA-BSA-水 混合液進行實驗時，所製得 MIP 可有對 BSA 高達 99％ 的選擇性，此值換算成吸附量為 6.370 mg-BSA/ g-MIP。由實驗結果可整理出此系統最佳合成條件如下：官能單體-交聯劑莫耳比：1：8，聚合溫度與時間：38℃ 與 60 小時。
第二種 MIP ，則是將其合成交聯劑由具線形分子結構的TEGDMA ，變更為非線形分子結構交聯劑：季戊四醇三丙烯酸酯 (Pentaerythritol tetraacrylate，PETTA)。此交聯劑，由於其分子結構為較不易轉動之硬鏈，因此所具有的構形變化自由度較 TEGDMA 低。故以此兩系統所得實驗結果進行比較後，可討論出交聯劑分子結構對所合成 MIP 影響性。經實驗後，可知其最佳合成條件為：官能單體-交聯劑莫耳比：1：11，聚合溫度與時間： 38℃ 與 48 小時。從實驗結果可知此系統所製得之 MIP 對 BSA 選擇性為 93.56％，並與前一系統數據相較後可認為：以線形分子結構交聯劑製作 BSA-MIP 應為較佳選擇。
以前述之 DMAPMA-TEGDMA MIP 合成系統進行其它方向的應用：如搭配石英微量天平 (Quartz crystal microbalance，QCM) 製作白蛋白感測器或使用微接觸 (Micro-contact imprintint) 技術製作 MIP 薄膜實驗等，亦都得到許多正面數據。除此之外，在以 SEM 與 AFM 進行 MIP 薄膜表面輪廓觀察時，亦成功觀察到 MIP 薄膜表面輪廓在吸附蛋白質前、後變化情形。

Molecularly imprinted polymers (MIPs) are synthetic macromolecular structures with selective recognition, determined mainly by the shapes of their cavities, and the chemical functionality imbedded in the matrix. MIPs show potential for application in many fields, e.g. sensor design, chromatograpic separation and catalysis.
Bovine serum albumin (BSA) was selected as an imprinting target for this study as it is a direct mammalian analogue of its human counterpart. Albumin is a major transport protein in blood and as such the development of synthetic matrices able to recognize this protein have potential application in diagnostics.
Two polymer formulations were used to form imprints of BSA. Firstly, a BSA-MIP was prepared with a linear cross-linker, i.e. 3-Dimethylaminopropyl methacrylamide-Tetraethyleneglycol dimethacrylate (DMAPMA-TEGDMA), in the presence of 6.04％ water. This polymer on re-binding showed a high selectivity (99％) towards its template molecule, corresponding to 6.370 mg-BSA/ g-MIP. From the experimental data, the optimum mole ratio of DMAPMA to TEGDMA, was 1：8, the optimal polymerization time was 60 hr with the synthesis being carried out at 38℃.
In the second approach, TEGDMA was replaced with Pentaerythritol tetraacrylate (PETTA), which is a non-linear cross-linking agent with a more rigid structure than TEGDMA therefore allowing less conformational freedom during MIP formation. From the experimental data, the optimum synthesis conditions of this system were determined as 48 hr polymerization at 38℃ and the mole ratio of DMAPMA to PETTA was 1：11. This MIP, formulated with DMAPMA and PETTA, showed a 93.56％ selectivity to BSA with a similar adsorption ability as the DMAPMA-TEGDMA co-polymer. Based on this result, we may deduce that the linear cross-linker is better optimised for preparation of the BSA imprinted polymer.
Application of the DMAPMA-TEGDMA MIP in different settings, such as in: sensors or thin film fabrication, gave good data. The MIP-QCM sensor showed excellent selectivity towards BSA. Synthesis of MIPs using micro-contact imprinting also showed good results. Also included are SEM and AFM images of MIP thin films made by micro-contact imprinting showing their surface morphology.

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