本研究係探討以半連續泥漿法在不同操作條件下，利用甲苯為生長基質共代謝三氯乙烯之效應。
在半連續泥漿培養試驗中，於三氯乙烯濃度0.5 ppm時，分別添加濃度為10 ppm及20 ppm之甲苯進行生物共代謝。研究顯示，在未考慮吸附及洩漏等非生物性降解因素下，培養至第四個培養期程時(約經過14天)，T1菌對於三氯乙烯降解率可高達93.2 %及96.6 %，原生菌則可去除85.2 %及89.9 %之三氯乙烯。當甲苯濃度愈高，三氯乙烯降解率亦隨之提高，然甲苯降解率反而稍降。若以20 ppm甲苯共代謝0.5 ppm與1 ppm兩種不同濃度之三氯乙烯，後者之三氯乙烯降解率大為下降，分別為植種組79.9 %與未植種組68 %。維持甲苯及三氯乙烯濃度比例為20：1，但將二者濃度分別提高至66.6 ppm及3.33 ppm，T1菌與原生菌對三氯乙烯之降解率分別為79.2 %及73.5 %，達穩定狀態之時間也延長至約50天左右。此外，T1菌以甲苯共代謝三氯乙烯之效果較佳，但三氯乙烯及代謝產物的生物毒性確實會對T1菌造成傷害，導致降解率隨培養時間拉長而下降；原生菌則對環境較具緩衝能力，能將生物毒性之影響減到最小。
根據穩定期單一期程試驗結果及微生物生長曲線顯示，T1菌及原生菌皆能在半天內使菌數及生物活性達最大。甲苯僅需約12小時即可降解90 %左右，三氯乙烯降解則發生於甲苯之後；主要原因為分解甲苯之同時才能產生可共代謝三氯乙烯之甲苯氧化酵素，且三氯乙烯競爭酵素之能力不如甲苯所致。
此外，本研究中之三氯乙烯及甲苯非生物性降解率(吸附及洩漏)分別為23.5 %及28.9 %。若將其列入計算，則在穩態下，三氯乙烯之實際生物降解則分別為植種組51.9 %～74.7 %及未植種組45.2 %～68 %。

The study focused on the toluene degraders on the cometabolic removal of trichloroethylene (TCE) with semicontinuous slurry microcosms method in different operating conditions.
In the semicontinuous slurry microcosm, initial TCE concentration was 0.5 ppm and toluene was used as primary substrate with addition in concentration of 10 ppm and 20 ppm, respectively. The results showed that the incubation periods were 14 days for the fourth incubation, the TCE removal efficiency with T1 strain were 93.2 % and 96.6 %, and with indigenous strains were 85.2 % and 89.9 % when the non-biodegradable factors such as adsorption and leakage were neglected. The TCE removal rate increased with an increase in the concentration of toluene. And the initial TCE removal rate of seeding and unseeding were 79.9 % and 68 % when the ratio of toluene and TCE concentration were 20:1. If the toluene and TCE concentration increased to 66.6 ppm and 3.33 ppm, the degraded rate of T1 and indigenous strains were 79.2 % and 73.5 % and reached a steady state after 50 days. Therefore, it could get better cometabolic removal of TCE with T1 strains when toluene was used as primary substrate, but the biotoxicity of cometabolic by-products certainly damage the T1 strain with the result that degrade rate decreased with an increase in the incubation periods. On the contrary, the indigenous strains had the better buffering capacity to environment, so the biotoxicity could decrease to minimum.
The results of single period and microbial growth curve in the steady state indicated T1 and indigenous strains reached the maximum bioactivity in half of the day. Toluene removal efficiency could reach almost 90 % after 12 hours, and the degradation of TCE follow to occur. The main reason was when toluene removal, the toluene oxygenase which could cometabolic removal of TCE produce simultaneously and conduce to the competition capacity of enzyme for TCE was weaker than toluene.
Furthermore, the non-biodegradable amounts (include adsorption and leakage) of TCE and toluene in the study were about 23.5 % and 28.9 %. If the part was to be incorporated to calculate, the actual biodegradable amounts of TCE of seeding and unseeding in steady state were 51.9 %-74.7 % and 45.2 %-68 %, respectively.

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