The objectives of this thesis conception are to enhance the activation and collection procedure of spermatozoa within the microfluidic environment. This study has selected zebrafish as an animal model due to their experiment-friendly characteristics and increased laboratory demand during the past decades. Two types of microfluidic devices such as 1) artificial cilia based serpentine microfluidic device and 2) microchannel embedded with fin structures are proposed for zebrafish sperm activation and sperm collection respectively. Proposed artificial cilia based serpentine microfluidic device is based on the global and rotation in the flow field resulting in the efficient mixing fluid within a short period ultimately resulting a sperm activation in quick succession. It demonstrates a 74 % of zebrafish sperm activation at the first 15 sec, which is utmost 20% better than that of the previously proposed methods of sperm activation. Moreover, microchannel for sperm collection is designed with the fin structure sperm retainers has efficiently collected about on an average number of 23 motile sperms within the sperm retainer, with given different experimental condition. In addition, microfluidics considered herein can be implemented for sperm activation and collection for the species with higher economic values for the process of In-Vitro Fertilization (IVF).

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