本論文以微波輔助綠色合成法製備金奈米粒子綴飾之還原氧化石 墨烯(Au/rGO)奈米複合物,並進一步沈積於網版印刷碳電極(SPCE)上製 得 Au/rGO/SPCE,用於葡萄糖與半胱胺酸的電化學感測。使用左旋精胺 酸作為還原劑,可在微波照射下將四氯金酸與氧化石墨烯同時還原形成 精胺酸被覆之 Au/rGO 奈米複合物。藉由穿透式電子顯微鏡,可觀察到 平均粒徑約 16.2±2.4 nm 的金奈米粒子均勻地綴飾在 rGO 的表面。利用 Au/rGO/SPCE 在 0.1 M 氫氧化鈉溶液中電化學偵測葡萄糖,計時安培法 顯示葡萄糖濃度有兩段線性範圍:0.1 ~ 0.9 mM 及 0.9 ~ 9 mM,其偵測 極限為 0.01 mM。而利用 Au/rGO/SPCE 在 0.1 M、pH 7.4 的磷酸鹽溶液 中偵測半胱胺酸,發現抗壞血酸及尿酸所引起的干擾率小於 18%,顯示 Au/rGO/SPCE 對半胱胺酸的偵測具有良好的選擇性。計時安培法顯示半 胱胺酸濃度有兩段線性範圍:0.5 ~ 4 μM 及 4 ~ 38 μM,其偵測極限為 0.05 μM。更者,藉由恆電位法所進行的動力學探討可得其速率常數(kcat) 及擴散係數(D)分別為 2.34×104 L/mol‧s 與 2.9×10-6 cm2/s。

In this study, Au nanoparticles-decorated reduced graphene oxide (Au/rGO) nanocomposite was prepared via a microwave-assisted green synthesis method, and further deposited on the screen printed carbon electrode (SPCE) to obtain the Au/rGO/SPCE for the electrochemical detection of glucose and L-cysteine. By using L-arginine as the reducing agent, chloroauric acid and graphene oxide were simultaneously reduced to form the arginine-capped Au/rGO nanocomposite under microwave irradiation. By transmission electron microscopy (TEM), it was observed that Au nanoparticles with a mean diameter of 16.2±2.4 nm were uniformly decorated on the surface of rGO. For the electrochemical detection of glucose in 0.1 M NaOH by the Au/rGO/SPCE, the amperometry result presented two linear ranges: 0.1 ~ 0.9 mM and 0.9 ~ 9 mM. The limit of detection (LOD) was 0.01 mM. For the L-cysteine detection in 0.1 M phosphate solution at pH 7.4, it was found that the interference response owing to the presence of ascorbic acid (AA) and uric acid (UA) was less than 18%, revealing the good selectivity of Au/rGO/SPCE for the detection of L-cysteine. The amperometry result indicated two linear ranges: 0.5 ~ 4 μM and 4 ~ 38 μM. The LOD was 0.05 μM. Furthermore, from the kinetic study by chronoamperometry, the rate constant (kobs) and diffusion coefficient (D) were obtained as 2.34×10-4 L/mol‧s and 2.9×10-6 cm2/s, respectively.

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