二氧化鈦中的氧空位因為擁有了可以改變二氧化鈦本身的光學和其他物化性質的能力，而在近幾年來開始有廣泛的研究探討。當氧空位參雜在二氧化鈦中時，二氧化鈦的能隙將被縮減，進而讓只能使用紫外光觸發光觸媒的二氧化鈦擁有了可見光光觸媒的能力。可見光光觸媒已被應用在許多的領域當中，其中包括了利用可見光光觸媒作為抗菌以及毒殺癌細胞的手段。除此之外，當二氧化鈦表面具有氧空位缺陷時，氧空位將會有助於二氧化鈦吸附空氣中的水或者是氧氣，造成二氧化鈦表面帶有較多的OH基而擁有較高的親水性。而在其他文獻中也直接指出，氧空位此種表面缺陷可吸附較多的有機離子或者是蛋白質，創造出一個細胞較利於生長的表面。即便已經有相當多的文獻探討細胞與氧空位的關係，但是一個較為全面的研究卻是尚未提出。本實驗假設了可見光的照射與否是二氧化鈦表面氧空位對於細胞最主要的影響因子，以自製的LED光照系統在細胞培養於基材上時作為光線來源，並且調節不同的照射參數。由實驗結果可以知道，細胞生長於基材上的表現可以藉由基材上的氧空位濃度調節以及可見光的照射參數改變(改變照射光強度或是照射波長)來進行調控。這個發現讓吾人可以藉由不同光照的調節，使得培養於具有氧空位的二氧化鈦基材上的細胞走向不同的命運，對於之後二氧化鈦作為生醫材料的應用上，將可以做為一個有用的參考。

Oxygen vacancy in titanium dioxide has been studied in numerous studies, due to the effects on the optical and electro properties of TiO2. It is shown that oxygen vacancy can narrow the band gap of TiO2 by acting as a dopant, and enables visible light to trigger the photocatalysis effects. It is overt that cells can easily be killed by the effect of TiO2 photocatalyst reaction. In the other hand, oxygen vacancy is one kind of surface defects of TiO2, it is reported that the defect sites on the surface have the ability to adsorb organic molecules, protein or water molecules, leading a cell-favoring surface. Thus, the actual interactions between TiO2 with oxygen vacancy and cells remain unknown. In this study, we presumed that visible light irradiated is the main mechanism to adjust cell performance on the TiO2 substrate with oxygen vacancies. The white LEDs were used as light source, giving different conditions when cell is cultured on the specimens. This study successfully demonstrates that the effects of oxygen vacancies on cells can adjust by varying the oxygen vacancy density or the light source, including changing the intensities and wavelength of light. It is of interest to apply these results to other TiO2 applications to directs cell fate.

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