人工植牙是近年主要治療缺牙症狀的方法，由鈦為材料製成的牙根可提供長期的穩定性與媲美真牙的咬合力。許多研究指出植體表面的粗糙度、結構尺寸以及化學性質對於蛋白質吸附有相當重要的影響，而蛋白質的吸附有助於骨細胞與植體間的結合，良好的結合有助於減少術後發炎的程度並降低日後植牙失敗的機會。本研究乃藉由飛秒雷射表面處理使植體形成奈米尺度的結構化表面，而此表面的物化性對於骨整合效應會具有關鍵性的影響。
研究中首先利用飛秒雷射實驗系統於純鈦基材上進行加工參數最佳化，探討不同的雷射功率、光班尺寸、與脈衝次數於鈦基板上所形成誘導性週期結構的變化。利用不同的脈衝次數進行加工可以於相同面積下獲得不同尺寸大小的表面結構與形貌；隨著脈衝次數的增加，加工表面所形成的條紋狀結構的完整性與表面起伏亦會有所變化。第二部分則是以前一部分獲得之參數進行大面積連續加工，利用不同脈衝次數的加工可獲得具有次微米尺度的誘導週期結構與具有微米尺度的孔洞與島狀結構，加工表面粗糙度亦會隨著脈衝次數的增加而上升。研究分析結果得知，加工基板表面有氧化層形成，此氧化層厚度會隨著脈衝次數增加而提升，在150次脈衝下其厚度可達100nm。以大於100次的脈衝條件下加工基材表面會有一氧化鈦相形成，隨著氧化層的厚度與一氧化鈦晶量的增加，基材表面的親水性質有明顯的提升並能促進磷灰石的生成與吸附。

Artificial dental implants are recently used as a main treatment of tooth loss. Dental implants made of titanium may provide long-term stability and occlusion like real ones. According to late researches, the roughness, size of surface structuring and chemical properties is significantly effective for protein adhesion, which is beneficial to the apposition between osteoblast to the implants. Good osseointegration may reduce inflammation after surgery and avoid the chances to failure. Nano-scale structurization on implant surface was achieved via femtosecond surface treatment. Properties of surface are the key factor with osseointegration in this study. In this experiments treatment on titanium substrate within areas are carried out via femtosecond laser and need to be investigated. From analysis by SEM and AFM the surface morphology are obtained with different treatment by various pulse numbers and roughness increased with the pulse numbers. From XRD analysis it is clear that substrate surface formed titanium monoxide when applied pulses were higher than 100. Contact angle measurement of surfaces treated by various pulses showed that the change of surface morphology provided improvement on wettability of treated surfaces.

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