質譜技術與蛋白質晶片技術已成為研究蛋白質體學的重要方法。蛋白質晶片技術可以高效地獲取生物體中的蛋白質訊息，且一次大量的樣品檢驗可減少所需成本。奈米粒子本身具有高的表面積及可與生物分子相容的優點，因此，在本研究中，以金奈米粒子修飾於聚雙甲基矽氧烷基材上，發展以此為基材之反相蛋白質陣列晶片。
我們成功地以血紅素蛋白質做為連結試劑，將金奈米粒子穩定地修飾於晶片表面上，首先針對雌激素受體ERα分析，可得到檢量線 ，線性範圍為44.6至1.67 ng/mL，求得此系統之偵測極限值為0.5 ng/mL。
針對所使用的抗體，也可以藉著反相蛋白質陣列晶片進行快速地篩選，並評估其專一性。更進一步，利用此晶片可以研究MCF-7細胞經過雌激素E2作用後，在不同時間點的蛋白質表現。使用修飾之PDMS基材製作組織晶片，可使組織平整貼附，且具有耐熱、耐有機溶劑等特性。
綜合以上實驗結果，顯示出以金奈米粒子修飾之聚雙甲基矽氧烷為基材之反相蛋白質陣列晶片，具有良好的再現性與穩定性，使用修飾後的PDMS基材製作組織晶片，也有極大的潛力。

Mass spectrometry and protein chip technology have already become important methods for proteomic research. Protein information from organisms can be gained high-efficiently by using protein chip technology. A large number of samples could be screened using protein chips at low cost. On the other hand, nanoparticles have many advantages such as large surface area and high biocompatibility. In this study, we would like to take these advantages associated with gold nanoparticles (AuNPs) in fabricating a reverse-phase protein array on AuNPs-anchored poly (dimethylsiloxane) (PDMS) substrate.
We successfully stabilized and immobilized Au nanoparticles on PDMS surface by using myoglobin as a linking agent. Recombinant estrogen receptor alpha (ERα) was first used to test our system. The calibration curve was estimated to be y=1.1451x + 2.4936 (R2=0.9954) in a linear range from 44.6 to 1.67 ng/mL and the detection limit was determined to be around 0.5 ng/mL.
We demonstrated that such modified PDMS chip could differentiate the specificity of antibodies from different sources at a high screening speed. Furthermore, we chip could be used for protein expression profiling of MCF-7 cells under 17β-estradiol (E2) treatment of different time courses. We further demonstrated that such modified PDMS chip could be used to immobilize tissue pieces smoothly and with good tolerance for heat and organic solvents.
Thus, the results confirmed that our reverse-phase protein array fabricated on AuNP-anchored PDMS substrate exhibited good reproducibility and stability. Furthermore, such modified PDMS chip holds a great potential to fabricate tissue arrays.

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