本實驗主要在研究硬脂酸( SA )/硬脂胺( ODA )混合單分子層系統與副相中葡萄糖氧化酵素( GOx )間特性的探討，可由吸附曲線得知，單分子層的存在使得GOx分子有兩個階段的吸附平衡，當改變不同比例的單分子層時，會影響到達平衡的速率及第二個平衡階段的平衡膜壓。藉由等溫線的操作及吸附行為可以知道GOx分子在第二個平衡階段的吸附量較第一個平衡階段多。而且從遲滯曲線及鬆弛行為可以觀察到在第一個平衡階段的吸附平衡裡，氣/液界面上的GOx分子並無達到吸附的飽和狀態，然而第二個平衡階段的吸附平衡則可由實驗數據顯示，在氣/液界面上的GOx分子已達到吸附的飽和狀態。鬆弛行為也可說明單分子層的存在，的確可以增加GOx分子在氣/液界面上的穩定性。利用電化學領域中的循環伏安法( Cyclic Voltammetry )分析沉積在固體基板( substrate )上的SA/ODA/GOx複合膜，可從測定開路電壓( OCP )對時間( t )的關係圖觀察到GOx分子的活性，並看出確實有酵素催化反應的進行，藉由反應電流( I )對時間( t )的數據圖知道，沉積的次數與葡萄糖溶液的濃度確實會影響反應電流發生的多寡。

In this study , the main theme is to examine the relationship between the mix-monolayer of stearic acid (SA) and octadecylamine (ODA), and the glucose oxidase (GOx) in subphase . Because the exist of monolayers , there are two equilibrium stage in the adsorption curve for GOx . If we changed the composition of monolayer , the rate of reaching the equilibrium stage and the equilibrium pressure of the second stage also changed. Isotherm and adsorption curve show that the quantity of adsorbed GOx in the first equilibrium stage is more than that the second equilibrium stage . According to the results of relaxation and hysteresis , the adsorption of GOx does not attain the saturated status at the first equilibrium stage . However , the adsorption behavior of GOx at the second equilibrium stage seems to attain the saturated status . The figure of relaxation also interepted that the exist of a monolayer indeed stabilize GOx molecules on air/water interface . We also use cyclic voltammetry to determine the electrochemical activity of the immobile GOx . The curve of open circular potential versus time reveals the catalytic reaction of enzyme ideed happened . Furthermore the response current is affected by the concerntration of glucose in electrolyte and the number of complex on working electrode .

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