在這篇論文中我們建構了兩種光學方法去探討與量測仿生物組織的假體光學性質，此兩種光學架構分別為頻域光子遷移系統搭配平面光架構(PSI)，以及頻域光子遷移系統搭配使用平面光的擴散探頭(DPPS)。其光源使用波長808奈米的近紅外光雷射，並利用光傳播理論模型去精確地得知組織的吸收係數和散射係數。在本實驗架構裡，首先，我們使用蒙地卡羅方法評估兩套架構之擴散理論表現及正確性;其次，測試了頻域光子遷移系統分別搭配兩種光源架構的振幅和相位的穩定性，這可以使我們了解系統的性能以及限制;再來我們使用兩套架構量測六種不同吸收和不同散射的仿生物組織之液態假體，並討論其表現與分析其優缺點;最後，驗證了此兩套架構:頻域光子遷移系統搭配平面光架構(PSI)，以及頻域光子遷移系統搭配使用平面光的擴散探頭(DPPS)，皆可以非侵入式的方法快速的量測到待測組織的光學參數。除此之外，不論在理論上或實驗上，我們亦證明了，搭配擴散探頭(DPPS)之方法優於搭配平面光架構(PSI)之方法，其可更精確定量樣品的光學參數。

In this thesis, we demonstrate the use of two optical methods, the frequency domain photon migration (FDPM) system configured in the planar source illumination (PSI) geometry and diffusing probe with planar source (DPPS) geometry, to investigate physiological parameters of biological tissues. The system uses near-infrared laser light (808nm) as light source and work in conjunction with mathematical photon transport models to accurately determine optical absorption (µa) and reduced scattering (µs′) properties of tissues. In this study, we would like to understand the performance of the PSI and DPPS geometries using numerical and phantom methods. First, we employ the Monte Carlo method to evaluate the performance of diffusion equations in PSI and DPPS geometries. Second, we characterize the stability of the amplitude and phase of the FDPM system configured in PSI and DPPS geometries so that we can understand the limitations of our systems. Third, we carry out PSI and DPPS measurements on six liquid phantoms of various absorption and scattering properties, and discuss the performance of two detected geometries and analyze their advantages and drawbacks. Finally, it is verified that the FDPM configured in the PSI and DPPS geometry provide a fast and noninvasive way to quantify optical properties of tissue. In addition, the DPPS method is more suitable and accurate for the determination of the optical properties of samples than the PSI method, either in the theory or in the experiments.

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