鈦金屬與鈦合金因為有生物惰性和優異的機械性能與骨頭有良好生物相容性，而廣泛應用於骨科和牙科。然而，在植入的早期階段，植體和周圍骨組織之間的骨整合不是很穩定，需要進一步改善。本研究所選擇的改質方法是藉由有機相電解液對材料進行陽極氧化，並經過真空熱處理後，製造出具可見光吸收能力二氧化鈦奈米管作為研究基材，並探討可見光的照射是否可激發二氧化鈦表面氧空位作用。氧空位是二氧化鈦中的重要缺陷，它在多種應用中起著至關重要的作用。當表面二氧化鈦層含有氧空位缺陷時，它會吸引空氣中的水並在頂部產生更多的OH基團，形成更高的親水表面，影響蛋白質在材料上的貼附及對細胞反應的影響。然而有文獻指出藍光會造成細胞活性氧化物(ROS)產生，我們在細胞培養液中加入輔酶Q10來降低細胞因為照光而產生的活性氧化(ROS)，期望能改善這個問題。
在材料分析方面，以SEM、EDS、XRD及PL等方法來分析經表面處理後的試片其物理及化學特性，再利用自製的LED系統作為光源並調節不同的照度來照射基材，進行細胞存活率分析(MTT)、鹼性磷酸酶(ALP)測試及活性氧(ROS)檢測實驗來觀察其上所培養的細胞表現。

Titanium implants have been widely used in orthopedic and dental applications due to their bioinert and excellent mechanical properties. However, in the early stage of implantation, osseointegration between the implant and surrounding bone tissue is not very stable and needs further improvement. The interaction between the native cells and the implant interface is a major factor in the clinical success of the implant.
In this study, flatter surfaces from macroscopic TiO2 nanotube arrays were created by glycerol-based electrolyte when compare to acidified electrolyte. Visible light sources were used as stimulation to the oxygen vacancies in the titanium dioxide layer to affect the protein attachment and hoping can guide the way of cell growth.
However, some studies demonstrated that blue light makes not only the bacterium apoptosis but cell dead. In addition, we added coenzyme Q10 in the cell culture medium to reduce the Reactive oxygen species (ROS) produced by the cells due to visible light.
Herein, we use SEM, EDS, XRD, PL and other equipments to analyze characterizations of the titanium discs after surface modification. Followed by MTT, ALP and ROS experiments to find out the cell performance after being seeding on surface modification titanium disc stimulates by self-assemble LED light source.

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