在拉曼增顯基材的製備上有許多不同的製備方法，而本論文研究包含兩個部分，第一部分是在不同基材上，藉由自組裝單分子層的方式將銀奈米粒方體自組裝於銀金屬薄膜上，探討銀金屬薄膜厚度變化對表面電將共振波長位置的改變，從實驗及FDTD模擬兩方面做為印證得到當銀金屬薄膜厚度到達一定值時，其拉曼增顯因子亦趨於一定值，並且在容易取得的鋁基材上也可以達到同樣的效果，因此我們可以藉由使用較少的時間及材料成本在基板的製備上達到相同的目的。
論文的第二部分則是承接第一部分取用便宜易取得的鋁基材以銀奈米立方體溶液滴定的方式，探討團聚效應對拉曼增顯的影響，並且與第一部分以自主裝方式製備的拉曼基板做比較，發現在以R6G、CV及4-ATP三種有機物做檢測，都可以觀察到滴式基板在偵測極限及增顯因子上都比以自組裝的拉曼基板要來的好，並且藉由FDTD模擬作為輔助說明除了奈米立方體與銀金屬薄膜間的共振腔形成的電磁場增顯外，奈米粒子間形成的熱點效應亦提供額外的電磁場增顯。

This research describes the application of positioning silver nanocubes on different substrates in Surface-Enhanced Raman Scattering (SERS). In the first section, self assembled monolayer(SAM) was used for substrate preparation to immobilize silver nanocubes on silver thin film. In order to knew the thickness effect of the silver thin films and their relationships with plasmonic resonance, silver thin films with different thickness were deposited on two different substrates. It was found that when the thickness of silver thin films reach certain value, their Raman enhancement factors(EF) are almost the same. Same phenomenon was also found on the aluminium substrate with silver thin films. From the result, we could prepare Raman substrate with less time and materials but with same Raman effect. In the second part, substrates were prepared by dropping silver nanocube solution on cheap and easily accessible aluminium substrate. By using three organic compounds R6G, CV and 4-ATP as analytes, silver nanocube solution dropping substrate obtain better Raman performance than the SAM substrate from both detection limit and EF. In order to understand this phenomenon, finite difference time domain method (FDTD) was used to simulate the local electric field intensity distribution. We find that the better performance of this substrate could be derived from the hot spots formation due to aggregation.

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