在本篇論文中我們架構頻域光子遷移系統的光學方法去量測生物組織的生理參數，它是使用波長808奈米的近紅外光並利用光傳播理論模型去精確地得知組織仿體的吸收係數和散射係數。在論文中我們使用頻域光子遷移系統並搭配自製探頭去量化仿體的光學性質，首先我們使用頻域光子遷移系統量測五種不同葡萄糖濃度的液態仿體，並量化液態仿體的吸收係數和散射係數，其次我們使用擴散理論模擬增加調變頻率的頻寬，進而證實增加調變頻率可以增加振幅及相位的解析度，再來我們使用Matlab撰寫新型縮放蒙地卡羅模型，相較於一般的蒙地卡羅演算法，速度快了將近五個數量級，且誤差率小於3%，相較於舊有的快速蒙地卡羅演算法，可以使用於更多光學參數的模擬而不影響模擬速度，最後我們使用新型縮放蒙地卡羅模型模擬波長1550奈米的光學參數，進而驗證我們撰寫的新型縮放蒙地卡羅模型其解析度為100 mg/dl，除此之外我們比較808奈米與1550奈米的模擬結果，提出同時使用808奈米與1550奈米波長光源的優勢，最後我們的研究結果驗證頻域光子遷移系統是一個能用來定量組織組成的快速非侵入式光學方法。

In this thesis, we demonstrate the use of optical method, frequency domain photon migration system (FDPM), to determine physiological parameters of biological tissues. It use a 808 nm laser diode as the light source coupled with mathematical photon transport models to accurately determine optical absorption (µa) and reduced scattering (µs′) properties of phantoms. Here, we employed the fiber probe with the FDPM technique to quantify the optical properties of in-vitro phantom. First, we use FDPM system to measure five glucose concentration liquid phantoms of various absorption and scattering properties. Second, we speculate that resolve the slight variation of optical properties is the advantage of the broad bandwidth FDPM system by using the diffusion model. Third, we implement the scaling Monte Carlo method by using Matlab. The scaling method can reduce computation time by nearly 5 orders of magnitude, and the error is less than 3% which is compared with the original Monte Carlo algorithm. The scaling Monte Carlo algorithm can be used obtain spatially resolved reflectance spectra of tissue with different optical parameters without significantly affecting simulation time. Finally, we speculate that resolve the slight variation of optical properties in scaling Monte Carlo method is 100 mg/dl. In addition, we combine the simulated result at 808 and 1550 nm with the specific advantage. Our study reveals that the FDPM system provides a fast and noninvasive way for tissue composition quantification.

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