陰陽離子液胞（catanionic vesicle）是一種新型的傳輸載體，利用陽離子型界面活性劑及陰離子型界面活性劑合成出類似脂質結構的離子對雙親分子，經過適當製程可製備出陰陽離子型液胞。本研究以雙碳鏈陽離子型界面活性劑dihexadecyldimethylammonium bromide（DHDAB）及單碳鏈陰離子型界面活性劑sodium dodecylsulfate（SDS）所形成之三碳鏈離子對雙親分子DHDA-DS為主材料，並添加膽固醇以強制性製程製備出陰陽離子液胞，探討稀釋及膽固醇的添加對於液胞物理特性及雙層膜流動性的影響。
實驗結果顯示，純成分DHDA-DS可形成帶正電之穩定液胞，且穩定天數可超過一年。而在製備液胞的過程中，水溶性較高之DS－會溶出雙層膜導致液胞帶正電。稀釋後液胞之界面電位下降，而以螢光偏極化法分析液胞雙層膜的流動性，顯示稀釋後液胞雙層膜流動性降低，兩者皆不利於液胞穩定。但當稀釋至0.033 mM（60倍）後，液胞仍可穩定超過半年，表示三碳鏈DHDA-DS液胞不易受到稀釋所造成的不穩定效應所影響。
添加膽固醇後，液胞之界面電位下降，顯示膽固醇可抑制DS－溶出雙層膜的行為。以螢光偏極化法及紅外光譜法分析雙層膜的流動性可知添加膽固醇後，靠近液胞雙層膜核心位置之碳鏈的流動性下降，但整體碳鏈的流動性上升，顯示膽固醇對於雙層膜的不同區段有不同的效應。位於膽固醇的固醇環周圍之DHDA-DS碳氫鏈分子傾向更規則的排列，稱為規則效應，而在DHDA-DS之碳氫鏈的前段及尾段部分為不規則效應，因此整體而言，膽固醇結構誘導出的不規則效應大於規則效應，使整體疏水碳鏈區域流動性上升。

In this study, the formation behavior of catanionic vesicles from a triple-chained ion pair amphiphile (IPA) was investigated by size, zeta potential, fluorescence polarization and infrared spectroscopy analyses. The IPA, dihexadecyldimethylammonium-dodecylsulfate (DHDA-DS), was obtained as precipitate from mixed aqueous solutions of anionic surfactant, sodium dodecylsulfate, and cationic surfactant, dihexadecyldimethylammonium bromide. Stable catanionic vesicles with a lifetime more than 360-day could be fabricated from DHDA-DS without additives. Whether the zeta potential of the catanionic vesicles decreased or the membrane rigidity of the catanionic vesicles increased, a detrimental effect on the physical stability of the catanionic vesicles would result. However, the vesicle size analysis indicated the high physical stability of the DHDA-DS catanionic vesicles even at a high dilution ratio. Fluorescence polarization and infrared spectroscopy showed that the addition of cholesterol into the DHDA-DS vesicles could have different effects on the vesicular membrane fluidity in different regions of the bilayer membranes, which was attributed to the difference in the extent of disordering and ordering effects induced by cholesterol.

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