本研究目的在探討以砂管柱連續流裝置，接種甲苯解菌，在進流甲苯10ppm及25ppm、不同水力停留時間(8.5、15及17小時)的操作條件下，對三氯乙烯0.5、1.0及3ppm之去除效率之影響，並尋求最佳操作條件。
　　第一階段探討限制因子(limiting factor)，第二階段繼續探討低濃度三氯乙烯共代謝去除效率，第三階段探討高濃度三氯乙烯共代謝去除效率，第四階段探討高低不同濃度甲苯造成之生物競爭基質效應，另外再探討甲苯分解菌當外來細菌，植入遭污染之管柱，進行優勢甲苯分解菌對系統共代謝三氯乙烯還原能力之研究。
　　第一階段管柱連續流進流20ppm甲苯(實測進流16.3-18.1ppm)、低濃度TCE 1ppm及0.5ppm(實測0.6-0.8ppm及0.29-0.38 ppm)，管柱出流菌落數約6x106 cfu，TCE濃度1ppm及0.5ppm對出流菌落數影響不大，對三氯乙烯去除率都可高達90%以上，兩段管柱總去除效果都可使三氯乙烯達到第二類法定標準0.05ppm以下(土壤污染防治法)，而在高濃度TCE試驗部分,進流3ppmTCE時(實測約在2.2ppm)，出流菌落數及三氯乙烯去除率有明顯之降低，菌落數約從6.0x106降至1.1x106cfu去除率在68-74%不等，且有越來越低之趨勢，高濃度TCE第二段管柱去除率約在35%下，推測是高濃度TCE產生較多之中間毒性產物，減低第二段生物活性，要使得排放濃度達到法定標準以下需往提高更多段之管柱或是提高更長水力停留時間。系統再活化之研究上，發現三氯乙烯去除率會因添加甲苯優勢菌有些微提高(從35%提升至45-50%)，顯然外來優勢菌添加有助於系統回復。在競爭基質研究上，發現進流10ppm(低基質)及25ppm(高基質)甲苯、三氯乙烯1ppm，其去除率初期(7天內)前者高達90%後者約在80%，但後期甲苯10ppm組別三氯乙烯去除率隨時間降低，在20天實驗約降到80%。

　　The purpose(s) of this study is to research the co-metabolism of trichloroethene (TCE) in the continuous reactor of sand column(s), growing the bacteria No.T1 which could metabolize toluene and co-metabolize TCE effectively. To discuss the best operational state(s) in co-metabolizing TCE of 0.5, 1.0 and 3.0 ppm under the hydraulic retention of time (HRT) in 8.5 hr and 17 hr, and the inflows concentration of toluene (substrate) are 20 and 25 ppm as followed the five steps.
　　In the first step we discover the limiting factor of co-metabolizing TCE in sand column is H2O2. In the second and third steps found that low concentration of TCE about 0.5ppm and 1ppm could be metabolism well in about 90% in two sections of columns, and the effluent concentration of TCE in about 0.05ppm or below it which is a statutory standard of Taiwan; but when the affluence of TCE is in high concentration about 2.3ppm, made the removal effect in about 68-74% or less and the effluence of bacterial counts decreased from 6×106 to almost 1.1×106 cfu/ml , and next section of column is 35%, it can be conjectured that poisonous intermediate(s) influenced the bio-active. In the step of competition in oxygenase of substrates found that inflow the low substrate of toluene in concentration of about 10 ppm can metabolize TCE superiorly than 25ppm and the effect are 90% and 80%. But after 7 days the superiority disappeared, it may because low concentration of substrate result in the bacterial active reducing. In the last step, when we paused the system for two day, the ability of co-metabolizing TCE change weaker with inflow the same concentration of toluene, and growing No.T1 bacteria to the sand column again, the system can recover in several level, recovering the effect from 35% to 45-50%.
　　The No. T1 bacteria can be utilized in bioremediation of groundwater, but it need further study to research the active of the bacteria T1 in in-situ field.

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