薑黃粉中之黃色色素薑黃素具有多種生物活性，被廣泛應用在抗發炎、抗癌症、細菌性疾病之治療。其中之薑黃色素類物質去甲氧基薑黃素 (DMC) 與雙去甲氧基薑黃素 (BDMC) 雖不及薑黃素，亦具有生物活性。雖然薑黃素具有治療性質，但由於它的低溶解性與低生物利用度，無法取得許可於臨床上使用。為了解決此限制，薑黃素的奈米化與包覆複合體製備已有廣泛之應用。本研究中，以三種薑黃色素類物質為原料，製備成奈米粒以甲殼素奈米粒包覆以及薑黃素C3以不同比例之α, β-環糊精製成包覆複合體。這些複合體以核磁共振儀、傅立葉轉換紅外線光譜儀、X-射線繞射儀與穿透式電子顯微鏡鑑定其性質。在體外試驗中，薑黃素奈米粒與包覆複合體分別有90.4與97.8% 之誘捕效率。薑黃素C3奈米粒與包覆複合體都顯示有更佳之抗氧化活性以及對革蘭氏陽性與陰性菌之有較佳之生長抑制性。α, β-環糊精包覆複合體顯示為薑黃素較佳之載體，因此可應用於細菌感染之治療

Curcumin, the bright yellow pigment of turmeric (Curcuma longa) possesses pleiotropic medicinal properties that have been widely used in the treatments of inflammatory diseases, cancer, bacterial diseases etc., Apart from curcumin, other curcumionoids, mainly demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) also show medicinal properties but not as effective as curcumin. Despite the therapeutic properties, curcumin and curcuminoids are not approved for clinical use due to its poor solubility and low bioavailability. To overcome this limitation, nanoparticles and inclusion complexes of curcumin are widely used. In this study, we used curcumin-C3 complex, a combination of all three curcuminoids as starting material and used two different approaches to test its effectiveness. Firstly, nanoparticles of curcumin and curcumin-C3 were prepared and then these nanoparticles were encapsulated in chitosan. Secondly, the curcumin and curcumin-C3 were combined with α and β-CD to form inclusion complexes using different molar ratios of cyclodextrins (1:1, 1:3, 1:5). These nanoparticles and inclusion complexes were characterized in both solid and solution forms by nuclear magnetic resonance, Fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy. The nanoparticles were characterized by nanoparticle tracking analysis. Additionally, in vitro assays using curcumin-C3 nanoparticles and inclusion complexes showed improved entrapment efficiency of 90.4% and 97.8% respectively. Our findings suggest that curcumin-C3 nanoparticle are effective than native curcumin. For inclusion complexes, α-CD at molar ratio 1:3 and 1:5 is suitable carrier for curcumin-C3. Compared to curcumin, curcumin-C3 shows more potency due to synergistic effect of three curcuminoids.

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