本研究動機在於開發酵素動態動力分割製程，自外消旋胺獲得其高純度鏡像異構醯胺。在研究方向上分成兩大部分，其一為以(S)-(α)-苯基乙胺及苯乙酮為反應基質，合成(S)-亞胺，並探討有機鹼觸媒、反應物濃度、有機溶劑種類及反應溫度等操作變因對消旋反應之影響。初步實驗結果顯示並未獲得外消旋亞胺。另一部分則以外消旋(α)-苯基乙胺為反應基質，進行酯類的篩選以及探討溫度、反應物濃度和有機溶劑對酵素選擇性之影響。實驗發現在100℃以下之正癸烷中，以甲氧基乙酸乙酯與外消旋(α)-苯基乙胺進行酵素動力分割反應，可獲得較佳的鏡像比值（E = 21.1）；以5 mM外消旋 (α)-苯基乙胺與10 mM甲氧基乙酸乙酯進行酵素動力分割，發現當溫度在70~80℃時，酵素有最佳之選擇性（E > 100）。

　　This research is aimed to develop an enzymatic dynamic kinetic resolution process, in which racemic (α)-arylamine is the substrate and a highly stereo-pure amide is obtained by employing an enzymatic amination coupled with in situ racemization of the remaining (S)-amine. The main works include racemization of (S)-(α)-phenylethylamine and enzymatic kinetic resolution of (R,S)-(α)-phenylethylamine. For the former, we synthesized and purified the (S)-imine synthesized from (S)-(α)-phenylethylamine and acetophone. Then, effects of the operation parameters such as organic bases as catalysts, reactant concentration, solvents and temperature were studied. Unfortunately, no 1, 3-proton shift reaction for the (S)-imine occurred. For the latter, the kinetic resolution of racemic (α)-phenylethylamine were investigated, in which esters, temperature, amine and ester concentrations were varied. The results indicate that with racemic (α)-phenylethylamine and ethyl methoxyacetate as substrates, high lipase enantioselectivity ( E > 100) was obtained at the temperature range of 70~80℃ in decane.

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王莉雯：外消旋suprofen硫酯之動態動力分割製程開發。國立成功大學碩士論文，2002。