本研究利用單寧酸與聚乙烯亞胺與四氯金酸在水的溶劑下進行一鍋合成法反應。單寧酸具有的金屬離子還原劑的效果，高分子聚乙烯亞胺可以做為保護劑，製作出沉澱物的奈米金顆粒與上清液的螢光奈米顆粒的產物，並透過不同濃度的聚乙烯亞胺控制奈米金顆粒的形狀與尺寸變化。將合成的奈米金藉由拉曼光譜的量測，並對照TEM圖的奈米粒子判斷，發現具有尖刺形狀的參數都有較好的放大訊號，確定是熱點效應讓訊號有強烈的放大，除此之外我們從不同奈米粒子的大小判斷出粒徑比較大的奈米粒子放大訊號會比其他的好，因此可以藉由控制粒子形狀與大小可以增強SERS效果。最後發現分支PEI中的Au@TNA/PEI:0.175 mM參數具有最好的SERS效果，我們期望可以將其應用在生物影像，藉由近紅外光的照射產生的電磁場放大，亞甲基藍分子原本微弱的拉曼訊號也跟著增強，最後可以在生物體內的環境被偵測到，達到追蹤與標定的效果。
除了奈米金顆粒外，我們發現上清液具有螢光的特性，將其用365nm紫外光照射時會產生450 nm到500 nm的螢光，為了能夠與奈米金一起應用在生物影像，我們透過細胞生存率實驗找出適合的濃度與細胞培養，最後在螢光顯微鏡下可以判斷出上清液進入到細胞質裡面，產生有標記的效果。
未來希望能夠同時結合上清液與沉澱物的螢光與SERS特性應用在生物影像，達到雙影像系統的偵測，提供更好的解析度與減少誤差，改善單影像的不足。

We want to synthesize materials with two optical properties and apply them to biological image detection. Compared to single images, the use of dual image system can increase the resolution and reduce the misdiagnosis of the situation. So master this technology can make the image to judge more information, and improve the application of nano-particles. In my research, we use one-pot synthesis that tannic acid was reacted with polyethyleneimine and tetrachloroauric acid under the solvent of water. Finally, we made the product of the precipitated nano gold particles and the supernatant of the fluorescent nanoparticles. The synthesized nano gold was measured by Raman spectroscopy, finding that the parameters with spikes have a better amplification signal which come from hot spot. Beside, particle size larger nanoparticle amplification signal will be better than the other. So we can control the particle shape and size can enhance the SERS effect.
In addition to the nano-gold particles, we found that the supernatant has fluorescent property, it will produce 450 nm to 500 nm fluorescence by 365 nm light irradiating. Through the cell survival rate experiment to find the appropriate concentration and cell culture, and the fluorescence microscope can determine the supernatant into the cytoplasm, showing the effect of the supernatant with the tag.The future hope to be able to combine the supernatant with the fluorescence and SERS characteristics applied in the biological image, to achieve dual image system detection. To provide better resolution and reduce errors, improve the single image of the lack of.

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